ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/031/SRR13071731/SRR13071731.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/035/SRR13071735/SRR13071735.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/034/SRR13071734/SRR13071734.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/030/SRR13071730/SRR13071730.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/028/SRR13071728/SRR13071728.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/033/SRR13071733/SRR13071733.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/032/SRR13071732/SRR13071732.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR130/029/SRR13071729/SRR13071729.fastq.gzprimaryOK200GenomicsDSB Repair Metabolism Lab, The Francis Crick Institutehttps://www.ebi.ac.uk/ena/browser/view/PRJNA678869Mus musculusThe goal of this study is to assess the effect of the deletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in mouse embryonic fibroblasts. Overall design: We used RTEL1F/F mouse embryonic fibroblasts that upon infection with Cre adenoparticles delete Rtel1 and upon addition of doxycycline overexpress WT RNaseH1GFP (Rtel1F/F WT RNH1-GFP MEFs). Rtel1F/F WT RNH1-GFP MEFs were infected with GFP or Cre-GFP adenovirus, after 48 h doxycycline (2 μg/ml) was added and cells were collected after 48 h. RNA:DNA hybrids were pulled down using the DRIP protocol and DNA was isolated and send for sequencing.xref:PubMed:33357438ENAGene., RNA, Ribonucleic, 0904/17, DNS, (Deoxyribonucleotide)n, ribose nucleic acid, clone 2.39, protein complex, P62, DNAn+1, Proteins, ribonucleic acids, Si-11, RNS, protein, Double-Stranded, sci, qkr, protein-containing complex, l(3)S090417, MER3, Deoxyribonucleic acids, (Deoxyribonucleotide)n+m, Si-11-6, protein polypeptide chains, RP11-539G11.1, native protein, Deoxyribonucleic Acid, natural protein, polypeptide chain, SZ1, yeast nucleic acid, Ribonukleinsaeure, KH93F, Protein, Gene Products, HOW, How, pentosenucleic acids, Ribonucleic acids, SEC63D1, ds-DNA, protein aggregate, desoxyribose nucleic acid, Non Polyadenylated, l(3)j5D5, RNA Gene Products, who, 24B, ribonucleic acid, Acid, thymus nucleic acid, l(3)s2612, Genomes, Non Polyadenylated RNA, Non-Polyadenylated, stru, Double Stranded, Deoxyribonucleic acid, proteins, Ribonucleic Acid, whole genome, Who/How, l(3)S053606, CG10293, Non-Polyadenylated RNA, Protein Gene Products, l(3)j5B5, Gene Proteins, DEL, ds DNA, DmelCG10293, Desoxyribonukleinsaeure, qkr[93F], anon-EST:Liang-2.39, Double-Stranded DNA, (Deoxyribonucleotide)m, DNA, deoxyribonucleic acids, DNAn, helicaseGene., RNA, Ribonucleic, 0904/17, DNS, (Deoxyribonucleotide)n, ribose nucleic acid, clone 2.39, protein complex, P62, DNAn+1, Proteins, ribonucleic acids, Si-11, RNS, protein, Double-Stranded, sci, qkr, protein-containing complex, l(3)S090417, MER3, Deoxyribonucleic acids, (Deoxyribonucleotide)n+m, Si-11-6, protein polypeptide chains, RP11-539G11.1, native protein, Deoxyribonucleic Acid, natural protein, polypeptide chain, SZ1, yeast nucleic acid, Ribonukleinsaeure, KH93F, Protein, Gene Products, HOW, How, pentosenucleic acids, Ribonucleic acids, SEC63D1, ds-DNA, protein aggregate, desoxyribose nucleic acid, Non Polyadenylated, l(3)j5D5, RNA Gene Products, who, 24B, ribonucleic acid, Acid, thymus nucleic acid, l(3)s2612, Genomes, Non Polyadenylated RNA, Non-Polyadenylated, stru, Double Stranded, Deoxyribonucleic acid, proteins, Ribonucleic Acid, whole genome, Who/How, l(3)S053606, CG10293, Non-Polyadenylated RNA, Protein Gene Products, l(3)j5B5, Gene Proteins, DEL, ds DNA, DmelCG10293, Desoxyribonukleinsaeure, qkr[93F], anon-EST:Liang-2.39, Double-Stranded DNA, (Deoxyribonucleotide)m, DNA, deoxyribonucleic acids, DNAn, helicasefalseDeletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in MEFsDeletion of the helicase RTEL1 in RNA:DNA hybrids throughout the genome and how this may be affected by overexpression of the protein WT RNaseH1GFP in MEFs2025-09-242021-01-06PRJNA678869GSE1615961009033357438