ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/091/SRR13127891/SRR13127891.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/090/SRR13127890/SRR13127890.fastq.gzprimaryOK200GenomicsThe University of Edinburghhttps://www.ebi.ac.uk/ena/browser/view/PRJNA680583Mus musculusxref:PubMed:36640365xref:PubMed:35858557Hematopoietic stem cell (HSC) generation in the aorta-gonads-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of these clinically important cells in vitro. Overall design: Single-cell transcriptome profiling of E11 AGM samples from PDGFRB +/+ (WT) (n=1) and PDGFRB -/- (KO) (n=1) miceENAfalsePDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogenyPDGFRβ+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny2025-09-242022-07-20PRJNA680583GSE162103100903664036535858557