ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/003/SRR13178503/SRR13178503_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/007/SRR13178507/SRR13178507_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/006/SRR13178506/SRR13178506_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/007/SRR13178507/SRR13178507_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/004/SRR13178504/SRR13178504_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/008/SRR13178508/SRR13178508_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/005/SRR13178505/SRR13178505_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/004/SRR13178504/SRR13178504_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/006/SRR13178506/SRR13178506_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/003/SRR13178503/SRR13178503_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/008/SRR13178508/SRR13178508_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR131/005/SRR13178505/SRR13178505_1.fastq.gzprimary200OKGenomicsState key laboratories of Agrobiotechnology, College of biological sciences, China Agricutural Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA681932Homo sapiensAberrant transcription in alveolar soft part sarcoma (ASPS) is orchestrated by the fusion oncoprotein, ASPSCR1-TFE3, product of a gene fusion created by a t(X 17) chromosomal translocation. Here, proteomics of proteins co-immunoprecipitating with ASPSCR1-TFE3 revealed strong enrichment of VCP/p97, an AAA+ ATPase with known segregase function. Positive and negative genetic experiments with ASPSCR1-TFE3 and VCP demonstrated that they function co-dependently for cancer cell proliferation, colony formation, enhancer activation, chromatin conformation, and transcription Overall design: ASPS cell lines, tumor from ASPS mouse model and tumor from ASPS patients are used for ASPSCR1, VCP, POLII and various histone modificationxref:PubMed:38326311ENAfalseASPSCR1-TFE3 reprograms transcription by organizing enhancers around hexameric VCP [hASPS_ChIP, ASPL_VCP_Pol2]ASPSCR1-TFE3 reprograms transcription by organizing enhancers around hexameric VCP [hASPS_ChIP, ASPL_VCP_Pol2]2025-09-242023-01-27PRJNA681932GSE162478960638326311