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key laboratories of Agrobiotechnology, College of biological sciences, China Agricutural University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA681940"],"scientific_name":["Homo sapiens"],"long_description":["ChIPseq experiments identify that ASPSCR1-TFE3 defines active enhancers across the genome and co-distributes with VCP/p97. RNAseq after knock-down of ASPSCR1-TFE3 or VCP, inhibition of VCP, or overexpression of each in alveolar soft part sarcoma cell lines demonstrates their co-dependence. HiChIP defines chromatin conformations that surround ASPSCR1-TFE3 targets and are lost on VCP knock-down. Overall design: ASPS cell lines, tumors from ASPS mouse model and tumors from ASPS patients are used for ChIP-seq with antibodies against ASPSCR1, VCP, POLII and various histone modifications. RNAseq in ASPS cell lines after application of siRNA against control, ASPSCR1-TFE3, or VCP, or application of VCP inhibitor CB-5083. H3K27ac-HiChIP in the FU-UR-1 cell line at baseline and after 6 days of siRNA against VCP."],"tag":["xref:PubMed:38326311"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"ASPSCR1-TFE3 reprograms transcription by organizing enhancers around hexameric VCP [Cell_lines_ChIP, FUUR1, 17934_FUUR1_KDs_H3K27ac]","description":"ASPSCR1-TFE3 reprograms transcription by organizing enhancers around hexameric VCP [Cell_lines_ChIP, FUUR1, 17934_FUUR1_KDs_H3K27ac]","dates":{"last_updated":"2025-09-24","first_public":"2020-12-16"},"accession":"PRJNA681940","cross_references":{"GEO":["GSE162480"],"taxon":["9606"],"PubMed":["38326311"]}}