ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/014/SRR13306514/SRR13306514_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/004/SRR13306504/SRR13306504_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/006/SRR13306506/SRR13306506_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/014/SRR13306514/SRR13306514_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/012/SRR13306512/SRR13306512_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/009/SRR13306509/SRR13306509_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/008/SRR13306508/SRR13306508_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/006/SRR13306506/SRR13306506_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/010/SRR13306510/SRR13306510_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/012/SRR13306512/SRR13306512_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/009/SRR13306509/SRR13306509_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/013/SRR13306513/SRR13306513_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/007/SRR13306507/SRR13306507_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/003/SRR13306503/SRR13306503_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/003/SRR13306503/SRR13306503_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/005/SRR13306505/SRR13306505_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/011/SRR13306511/SRR13306511_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/013/SRR13306513/SRR13306513_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/011/SRR13306511/SRR13306511_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/010/SRR13306510/SRR13306510_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/007/SRR13306507/SRR13306507_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/004/SRR13306504/SRR13306504_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/008/SRR13306508/SRR13306508_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR133/005/SRR13306505/SRR13306505_2.fastq.gzprimaryOK200GenomicsUniversity of Pittsburghhttps://www.ebi.ac.uk/ena/browser/view/PRJNA687768Mus musculusxref:PubMed:37107275FAN1 is a DNA endonuclease that we have previously identified as the underlying genetic cause of karyomegalic interstitial nephritis (OMIM: 614817) in humans. In order to deduce the molecular function of FAN1 in the setting of acute kidney injury and progression to chronic kidney disease, we generated a proximal tubule specific Fan1 knockout mouse model and subjected these mice to cisplatin injury. RNA-seq of the transgenic mice kidneys revealed dysregulation of the DNA damage repair pathway as well as cell cycle associated genes. Together, the data supports for a role of Fan1 in DNA damage repair, and that in its absence results in dysfunctional proximal tubule repair and regeneration upon cisplatin injury. Overall design: 2X150 bp standard RNA-seq was performed using an Illumina HiSeq platformENAfascin-1, Research Related Injuries, proximal kidney tubule, Platinol, cis-Diamminedichloroplatinum(II), cis-Dichlorodiammineplatinum(II), determination, Cismaplat, Randa, Wound, NSC-119875, Injury and Wounds, renal proximal tubule, p55, Physical, RNA-seq, (SP-4-2)-, Physical Traumas, MTMR15, cis Platinum, injury, proximal tubule, diamminedichloro-, Injuries and Wounds, trauma, HSN, KMIN, Wounds, traumatic injury, Wounds and Injury, Trauma, chemical analysis, (SP-4-2)-diamminedichloridoplatinum, Peyrone's chloride, KIAA1018, Whole Transcriptome Shotgun Sequencing, CDDP, Physical Trauma, Peyrone's salt, Research-Related, FAN1, cis-diammineplatinum(II) dichloride, Fan1, cis-diamminedichloroplatinum, cis-platin, cis-[PtCl2(NH3)2], AI663989, Injury, cis-diamminedichloroplatinum(II), cis-Diamminedichloroplatinum, cis-dichlorodiammineplatinum(II), cis-diamminedichloridoplatinum(II), Neoplatin, cis-DDP, SNL, Diamminodichloride, Dichlorodiammineplatinum, cis-Platinum, cis Diamminedichloroplatinum, [PtCl2(NH3)2], wound, Platinum Diamminodichloride, Platinum, cisplatine, Lederplatin, Briplatin, cisplatin, Injuries, Platamine, cisplatino, Injuries., Kidneys, proximal convoluted tubule, Traumas, Platidiam, Biocisplatinum, Cisplatin, Platino, (SP-4-2)-diamminedichloroplatinum, Research-Related Injuries, TG, cisplatinum, assay, Research-Related Injury, kidney proximal tubule, Platinexfascin-1, Research Related Injuries, proximal kidney tubule, Platinol, cis-Diamminedichloroplatinum(II), cis-Dichlorodiammineplatinum(II), determination, Cismaplat, Randa, Wound, NSC-119875, Injury and Wounds, renal proximal tubule, p55, Physical, RNA-seq, (SP-4-2)-, Physical Traumas, MTMR15, cis Platinum, injury, proximal tubule, diamminedichloro-, Injuries and Wounds, trauma, HSN, KMIN, Wounds, traumatic injury, Wounds and Injury, Trauma, chemical analysis, (SP-4-2)-diamminedichloridoplatinum, Peyrone's chloride, KIAA1018, Whole Transcriptome Shotgun Sequencing, CDDP, Physical Trauma, Peyrone's salt, Research-Related, FAN1, cis-diammineplatinum(II) dichloride, Fan1, cis-diamminedichloroplatinum, cis-platin, cis-[PtCl2(NH3)2], AI663989, Injury, cis-diamminedichloroplatinum(II), cis-Diamminedichloroplatinum, cis-dichlorodiammineplatinum(II), cis-diamminedichloridoplatinum(II), Neoplatin, cis-DDP, SNL, Diamminodichloride, Dichlorodiammineplatinum, cis-Platinum, cis Diamminedichloroplatinum, [PtCl2(NH3)2], wound, Platinum Diamminodichloride, Platinum, cisplatine, Lederplatin, Briplatin, cisplatin, Injuries, Platamine, cisplatino, Injuries., Kidneys, proximal convoluted tubule, Traumas, Platidiam, Biocisplatinum, Cisplatin, Platino, (SP-4-2)-diamminedichloroplatinum, Research-Related Injuries, TG, cisplatinum, assay, Research-Related Injury, kidney proximal tubule, PlatinexfalseRNA-seq analysis of proximal tubule specific Fan1 knockout transgenic kidneys subjected to cisplatin injuryRNA-seq analysis of proximal tubule specific Fan1 knockout transgenic kidneys subjected to cisplatin injury2025-09-242023-05-05PRJNA687768GSE1638621009037107275