{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/027/SRR13479127/SRR13479127_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/026/SRR13479126/SRR13479126_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/027/SRR13479127/SRR13479127_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR134/026/SRR13479126/SRR13479126_1.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Zhejiang University, College of Pharmaceutical Sciences"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA693030"],"scientific_name":["Mus musculus"],"long_description":["Analysis of mHSPCs infected with PML/RARa(WT) or PML/RARa(2KR: K227R and K360R in RARa moiety). We used RNA-sequencing to detail the global programme of gene expression in PML/RARa(2KR)-overexpressing mHSPCs. Results provide insight into the reduced clonogenicity caused by PML/RARa(2KR) Overall design: Bone marrow cells were first obtained from the femurs of 5-fluorouracil-treated C57BL/6 mice, and then mHSPCs were selected from bone marrow cells using the Easysep Mouse Hematopoietic Progenitor Cell Isolation Kit (Stemcell Technologies). mHSPCs were cultured in a six-well plate and exposed to concentrated lentivirus (pMSCV, PML/RARa(WT) or PML/RARa(2KR)) in the presence of 6 µg/ml of polybrene. Infection was obtained by centrifugation of the cells in viral supernatant at 1,800 rpm for 40 min. The cells were cultured for 24 h at 37 °C. The following day, the medium was removed and replaced with fresh culture medium. Transduced mHSPCs were sorted and collected for RNA extraction and RNA-sequencng."],"tag":["xref:PubMed:35194189"],"repository":["ENA"],"description_synonyms":["RAR, Mus musculus, multinuclear leukocyte, Laboratory, RNF71, Swiss, axis, mice, Mus domesticus, 1200009E24Rik, progressive multifocal, mouse, Swiss Mouse, RARalpha1, House Mice, Swiss Mice, stalk, Scanning Transmission, leukoencephalopathy, PP8675, rara2a, House Mouse, culm, domesticus, Laboratory Mice, MYL, Electron Microscopy, Nr1b1, Trim19, primary axis, House, Mus, AI661194, NR1B1, PML, STEM, Mus musculus domesticus, polymorphonuclear leucocyte, Scanning Transmission Electron Microscopy, Cell., Mouse, TRIM19, house mouse, primary stem, Mice, Laboratory Mouse, nr1b1"],"name_synonyms":["RAR, Mus musculus, multinuclear leukocyte, Laboratory, RNF71, Swiss, axis, mice, Mus domesticus, 1200009E24Rik, progressive multifocal, mouse, Swiss Mouse, RARalpha1, House Mice, Swiss Mice, stalk, Scanning Transmission, leukoencephalopathy, PP8675, rara2a, House Mouse, culm, domesticus, Laboratory Mice, MYL, Electron Microscopy, Nr1b1, Trim19, primary axis, House, Mus, AI661194, NR1B1, PML, STEM, Mus musculus domesticus, polymorphonuclear leucocyte, Scanning Transmission Electron Microscopy, Cell., Mouse, TRIM19, house mouse, primary stem, Mice, Laboratory Mouse, nr1b1"],"additional_accession":[]},"is_claimable":false,"name":"The effect of two lysines in PML/RARa on mouse haematopoietic stem/progenitor cells","description":"The effect of two lysines in PML/RARa on mouse haematopoietic stem/progenitor cells","dates":{"last_updated":"2025-09-24","first_public":"2022-02-19"},"accession":"PRJNA693030","cross_references":{"GEO":["GSE165046"],"taxon":["10090"],"PubMed":["35194189"]}}