ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR135/082/SRR13503882/SRR13503882.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR135/079/SRR13503879/SRR13503879.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR135/075/SRR13503875/SRR13503875.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR135/076/SRR13503876/SRR13503876.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR135/083/SRR13503883/SRR13503883.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR135/078/SRR13503878/SRR13503878.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR135/077/SRR13503877/SRR13503877.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR135/081/SRR13503881/SRR13503881.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR135/084/SRR13503884/SRR13503884.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR135/080/SRR13503880/SRR13503880.fastq.gzprimaryOK200GenomicsTU Dresdenhttps://www.ebi.ac.uk/ena/browser/view/PRJNA693993Conditional gene inactivation is a powerful tool to determine gene function when constitutive mutations result in detrimental effects. The most commonly used technique to achieve conditional gene inactivation employs the Cre/loxP system and its ability to delete DNA sequences flanked by two loxP sites. However, targeting a gene with two loxP sites is time and labor consuming. Here, we show Cre-Controlled CRISPR (3C) mutagenesis to circumvent these issues. 3C relies on gRNA and Cre-dependent Cas9-GFP expression from the same transgene. Exogenous or transgenic supply of Cre results in Cas9-GFP expression and subsequent mutagenesis of the gene of interest. The recombined cells become fluorescently visible enabling their isolation and subjection to various omics techniques. Hence, 3C mutagenesis provides a valuable alternative to the production of loxP-flanked alleles. It might even enable the conditional inactivation of multiple genes simultaneously and should be applicable to other model organisms amenable to single integration transgenesis.ENAzebra fish, Controlling, Zebra, leopard danio, Materials, Danio rerio, Zebra Fish, Genetic, reference sample, Danio frankei, mutagenesis, Zebrafishes, STK10, Gene, FASTK, INSDC_feature:gene, Fishes, danio, Brachydanio rerio frankei., Cistrons, Zebra danios, Fast, Zebra danio, zebra danio, ATP:Fas-activated serine/threonine protein phosphotransferase activity, Material, B. rerio, Danio rerio frankei, Zebra Fishes, Mutageneses, D. rerio, Genetic Materials, Brachydanio rerio, Cistron, Cyprinus rerio, zebrafish, FAST, Genetic Material, D. rerios, ControlledfalseCre-Controlled CRISPR mutagenesis: fast and easy conditional gene inactivation in zebrafish2023-05-192021-01-24PRJNA693993