{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR137/087/SRR13731687/SRR13731687.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR137/083/SRR13731683/SRR13731683.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR137/086/SRR13731686/SRR13731686.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR137/082/SRR13731682/SRR13731682.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR137/084/SRR13731684/SRR13731684.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR137/085/SRR13731685/SRR13731685.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR137/081/SRR13731681/SRR13731681.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Pathology, Sapporo Medical University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA702454"],"scientific_name":["Homo sapiens"],"long_description":["Recent studies revealed that treatment resistant cancer stem-like cells (CSCs)/cancer-initiating cells (CICs) can be targeted by cytotoxic T lymphocytes (CTLs). CTLs recognize antigenic peptide derived from tumor-associated antigens (TAAs), thus identification of tumor-associated antigens (TAAs) expressed in CSCs/CICs is essential. Human leucocyte antigen (HLA) ligandome analysis using mass spectrometry enables analysis of naturally expressed antigenic peptides however, HLA ligandome analysis requires large scale of sample and it is challenging for CSCs/CICs. In this study, we established novel bladder CSC/CIC model from a bladder cancer cell line UM-UC-3 cells using ALDEFLUOR assay. CSCs/CICs were isolated as aldehyde dehydrogenase (ALDH) high cells and several ALDHhigh clone cells were established. ALDHhigh clone cells were enriched with CSCs/CICs by sphere formation and tumorigenicity in immune deficient mouse. HLA ligandome analysis and gene expression (CAGE) using ALDHhigh clone cells revealed distinctive antigenic peptide repertoire in bladder CSCs/CICs, and we identified GRIK2 derived antigenic peptide is specifically expressed in CSCs/CICs. GRIK2 peptide-specific CTL clone recognized GRIK2-overexpressed UM-UC-3 cells and ALDHhigh clone cells indicating that GRIK2 peptide can be a novel target for bladder CSCs/CICs-targeting immunotherapy. Overall design: Urothelial CSCs/CICs were isolated by an ALDEFLUOR assay fron human urothelial carcinoma cells, UM-UC-3. Aldehyde dehydrogenase-high (ALDHhigh) clone cell were isolated as urothelial CSCs/CICs. ALDHlow clone cells were isolated as urothelial non-CSCs/CICs. ALDHhigh clones (H-1, H-6, H-10) were used as CSCs/CICs clones and ALDHlow clones (L-1, L-3, L-8) were used as non-CSCs/CICs. Wild type UM-UC-3 cells were used as a control."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"GRIK2 is a target for bladder cancer stem-like cell-targeting immunotherapy","description":"GRIK2 is a target for bladder cancer stem-like cell-targeting immunotherapy","dates":{"last_updated":"2025-09-24","first_public":"2021-10-03"},"accession":"PRJNA702454","cross_references":{"GEO":["GSE166947"],"taxon":["9606"]}}