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Leuven"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA704681"],"long_description":["Monocyte-derived macrophages (Mφs) are crucial regulators during muscularis inflammation. However, it is unclear which micro-environmental factors are responsible for monocyte recruitment and anti-inflammatory Mφ differentiation in this paradigm. Here, we investigate Mφ heterogeneity at different stages of muscularis inflammation and determine how environmental cues can attract and activate tissue-protective Mφs. Results showed that muscularis inflammation induced marked alterations in mononuclear phagocyte populations associated with a rapid infiltration of Ly6c+ monocytes that locally acquired unique transcriptional states. Trajectory inference analysis revealed two main pro-resolving Mφ subpopulations during the resolution of muscularis inflammation, i.e. Cd206+ MhcIIhi and Timp2+ MhcIIlo Mφs. Interestingly, we found that damage to the micro-environment upon muscularis inflammation resulted in EGC activation, which in turn stimulated monocyte infiltration and the consequent differentiation in anti-inflammatory CD206+ Mφs via CCL2 and CSF1, respectively. In addition, CSF1-CSF1R signaling was shown to be essential for the differentiation of monocytes into CD206+ Mφs and EGC proliferation during muscularis inflammation. Our study provides a comprehensive insight into pro-resolving Mφ differentiation and their regulators during muscularis inflammation. We deepened our understanding in the interaction between EGCs and Mφs, thereby highlighting pro-resolving Mφ differentiation as a potential novel therapeutic strategy for the treatment of intestinal inflammation. Overall design: Refer to individual Series"],"tag":["xref:PubMed:36071145"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Enteric glial cells favor accumulation of anti-inflammatory macrophages during the resolution of muscularis inflammation","description":"Enteric glial cells favor accumulation of anti-inflammatory macrophages during the resolution of muscularis inflammation","dates":{"last_updated":"2025-09-24","first_public":"2022-10-07"},"accession":"PRJNA704681","cross_references":{"GEO":["GSE167465"],"PubMed":["36071145"]}}