{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/098/SRR13893198/SRR13893198.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/004/SRR13893204/SRR13893204.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/000/SRR13893200/SRR13893200.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/097/SRR13893197/SRR13893197.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/099/SRR13893199/SRR13893199.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/003/SRR13893203/SRR13893203.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/096/SRR13893196/SRR13893196.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/002/SRR13893202/SRR13893202.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/005/SRR13893205/SRR13893205.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/001/SRR13893201/SRR13893201.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR138/095/SRR13893195/SRR13893195.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["University of Pennsylvania"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA707553"],"scientific_name":["Mus musculus"],"tag":["xref:PubMed:34648590"],"long_description":["Cell death plays a critical role in inflammatory responses. During pyroptosis, inflammatory caspases cleave Gasdermin D (GSDMD) to release an N-terminal fragment that generates plasma membrane pores that mediate cell lysis and IL-1 release. However, certain stimuli and cell types release IL-1 cytokines through GSDMD pores in cells that remain viable, a process termed hyperactivation. How these distinct cell fate choices are regulated downstream of GSDMD pore formation is unknown. Here, we demonstrate that the Card19 locus promotes lysis downstream of caspase activation. Despite being largely protected from cell death, CARD19-deficient macrophages had no defect in caspase activation, IL-1 secretion, or GSDMD cleavage. CARD19, a mitochondrial protein, was not responsible for the observed phenotypes, nor was the recently identified pore forming protein NINJ1 which regulates terminal pore formation during multiple forms of cell death. Furthermore, previously identified cell death phenotypes attributed to Sterile alpha and heat Armadillo motif-containing protein (SARM) were revealed to be caused by a passenger mutation. Importantly, Card19-/ mice had increased susceptibility to Yersinia infection, including increased mortality, higher bacterial burdens and pronounced splenomegaly. These findings implicate the Card19 locus as a factor that couples caspase processing and GSDMD cleavage to cell death, providing insight into how the checkpoint between hyperactivation and cell death is regulated.   Overall design: Total 11 samples. Three technical replicates were analyzed for four groups (two genotypes, with or without LPS). Sample 7 was lost during processing and was not included in analysis."],"repository":["ENA"],"description_synonyms":["2Ab17, Caspase-Dependent Apoptosis, Su(var)3-1, caspase-dependent programmed cell death, Inflammatory Apoptosis, Pyroptotic Cell Death, cleavage, Epidermal Cell Derived Thymocyte-Activating Factor, Caspase Dependent Apoptosis, Caspase 1 Dependent Cell Death, Jil-1, interleukin-1 receptor ligand, Su-var(3)103, mAb2A, IL-1, signaling (initiator) caspase activity, Pyroptoses, Macrophage Cell Factor, induction of apoptosis, Extrinsic Pathway Apoptoses, IL1-BETA, activation of caspase activity, Intrinsic Pathway, Extrinsic Pathway Apoptosis, Programmed Cell Death, anon-WO0118547.379, IL1F2, Caspase-Dependent, Type I, Death, IL-1B, Cell Deaths, DmelCG6297, Lymphocyte Activating Factor, Caspase-1 Dependent Cell Death, Su(var)3-103, JIL1, Jil1, apoptosis, Pyroptotic Cell, activation of metacaspase activity, Interleukin I, Pyroptotic Cell Deaths, pyroptosis, Cell Death, Interleukin 1, Deaths, apoptosis activator activity, il1b-A, Classic Apoptoses, Intrinsic Pathway Apoptoses, Cytokine., Intrinsic Pathway Apoptosis, MSK, Su-var(3)1, Epidermal Cell Derived Thymocyte Activating Factor, induction of apoptosis by p53, CG6297, Classic, Classic Apoptosis, Inflammatory Apoptoses, JIL, Classical Apoptosis, Extrinsic Pathway, commitment to apoptosis, Pyroptotic, Lymphocyte-Activating Factor, Inflammatory, Classical, Apoptoses, T Helper Factor, Apoptosis"],"name_synonyms":["2Ab17, Caspase-Dependent Apoptosis, Su(var)3-1, caspase-dependent programmed cell death, Inflammatory Apoptosis, Pyroptotic Cell Death, cleavage, Epidermal Cell Derived Thymocyte-Activating Factor, Caspase Dependent Apoptosis, Caspase 1 Dependent Cell Death, Jil-1, interleukin-1 receptor ligand, Su-var(3)103, mAb2A, IL-1, signaling (initiator) caspase activity, Pyroptoses, Macrophage Cell Factor, induction of apoptosis, Extrinsic Pathway Apoptoses, IL1-BETA, activation of caspase activity, Intrinsic Pathway, Extrinsic Pathway Apoptosis, Programmed Cell Death, anon-WO0118547.379, IL1F2, Caspase-Dependent, Type I, Death, IL-1B, Cell Deaths, DmelCG6297, Lymphocyte Activating Factor, Caspase-1 Dependent Cell Death, Su(var)3-103, JIL1, Jil1, apoptosis, Pyroptotic Cell, activation of metacaspase activity, Interleukin I, Pyroptotic Cell Deaths, pyroptosis, Cell Death, Interleukin 1, Deaths, apoptosis activator activity, il1b-A, Classic Apoptoses, Intrinsic Pathway Apoptoses, Cytokine., Intrinsic Pathway Apoptosis, MSK, Su-var(3)1, Epidermal Cell Derived Thymocyte Activating Factor, induction of apoptosis by p53, CG6297, Classic, Classic Apoptosis, Inflammatory Apoptoses, JIL, Classical Apoptosis, Extrinsic Pathway, commitment to apoptosis, Pyroptotic, Lymphocyte-Activating Factor, Inflammatory, Classical, Apoptoses, T Helper Factor, Apoptosis"],"additional_accession":[]},"is_claimable":false,"name":"The Card19 locus regulates apoptosis and pyroptosis downstream of caspase activation and gasdermin D cleavage independently from IL-1 cytokine release","description":"The Card19 locus regulates apoptosis and pyroptosis downstream of caspase activation and gasdermin D cleavage independently from IL-1 cytokine release","dates":{"last_updated":"2025-09-24","first_public":"2021-03-23"},"accession":"PRJNA707553","cross_references":{"GEO":["GSE168489"],"taxon":["10090"],"PubMed":["34648590"]}}