ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/095/SRR14041795/SRR14041795_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/095/SRR14041795/SRR14041795_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/094/SRR14041794/SRR14041794_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/096/SRR14041796/SRR14041796.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/097/SRR14041797/SRR14041797.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR140/094/SRR14041794/SRR14041794_2.fastq.gzprimaryOK200GenomicsDongchen's lab, Institute for Immunology and School of Medicine, Tsinghua Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA716539Mus musculusDuring germinal center (GC) reactions, activated B cells undergo clonal expansion and functional maturation to produce high-affinity antibodies, and differentiate into plasma and memory cells, accompanied with class-switching recombination (CSR) and somatic hypermutation (SHM). Activation-induced deaminase (AID) is responsible for both CSR and SHM in GC B cells. Transcriptional mechanisms underlying AID regulation and GC B cell reactions are still not well understood. Here, we show that expression of Ascl2 transcription factor is upregulated in GC B cells. Ectopic expression of Ascl2 promotes GC B cell development and enhances antibody production as well as affinity maturation. Conversely, deletion of Ascl2 in B cells impairs the germinal center response. Genome-wide analysis reveals that Ascl2 directly regulates GC B cell-related genes, including AID ectopic expression of AID in Ascl2-deficient B cells rescues their antibody defects. Thus, Ascl2 regulates AID transcription and promotes germinal center B cell responses. Overall design: We constructed RVKM-Ascl2 retrovirus, and performed RNA-seq using transfected in vitro cultured activated B cells. Also, ChIP-seq of GC B cells from draining LN of KLH immunized C57BL/6 mice.ENARNA polymerase II core promoter proximal region sequence-specific binding, ASH2, T-cell leukemia, B Cells, B Lymphocytes, Bursa-Dependent Lymphocytes, AGL4, bHLHa45, bacterial transcription., B cell, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, Factor, SEPALLATA 2, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, Transcription Factor, AID, Aid, Human Donor, transcription factor activity, Mash2, Donor Artificial Insemination, zinc ion regulated core promoter proximal region sequence-specific DNA binding, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, CDA2, RNA polymerase II transcription factor activity, Heterologous Insemination, MASH2, Donor, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, KNOTTED1-like homeobox gene 5, aid, Donor Artificial, Artificial, F14P3.4, Insemination, Transcription, B-cell, Factors, Arp2, ARP2, F14P3_4, F10N7_150, Bursa-Equivalent Lymphocyte, metal ion regulated core promoter proximal region sequence-specific binding, copper ion regulated core promoter proximal region sequence-specific binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, 2410083I15Rik, Transcription factor, F10N7.150, Artificial Insemination, B lymphocyte, RNA polymerase II proximal promoter sequence-specific DNA binding, B-Lymphocyte, HEL-S-284, copper ion regulated proximal promoter sequence-specific DNA binding, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, AGAMOUS-like 4, HASH2, HIGM2, zinc ion regulated proximal promoter sequence-specific DNA binding, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, B-lymphocyte, transcription from bacterial-type RNA polymerase promoter, metal ion regulated sequence-specific DNA binding, metal ion regulated proximal promoter sequence-specific DNA binding, TRANSCRIPTION FACTOR, Heterologous, RNA polymerase II distal enhancer sequence-specific bindingRNA polymerase II core promoter proximal region sequence-specific binding, ASH2, T-cell leukemia, B Cells, B Lymphocytes, Bursa-Dependent Lymphocytes, AGL4, bHLHa45, bacterial transcription., B cell, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, Factor, SEPALLATA 2, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, Transcription Factor, AID, Aid, Human Donor, transcription factor activity, Mash2, Donor Artificial Insemination, zinc ion regulated core promoter proximal region sequence-specific DNA binding, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, CDA2, RNA polymerase II transcription factor activity, Heterologous Insemination, MASH2, Donor, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, KNOTTED1-like homeobox gene 5, aid, Donor Artificial, Artificial, F14P3.4, Insemination, Transcription, B-cell, Factors, Arp2, ARP2, F14P3_4, F10N7_150, Bursa-Equivalent Lymphocyte, metal ion regulated core promoter proximal region sequence-specific binding, copper ion regulated core promoter proximal region sequence-specific binding, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, 2410083I15Rik, Transcription factor, F10N7.150, Artificial Insemination, B lymphocyte, RNA polymerase II proximal promoter sequence-specific DNA binding, B-Lymphocyte, HEL-S-284, copper ion regulated proximal promoter sequence-specific DNA binding, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, AGAMOUS-like 4, HASH2, HIGM2, zinc ion regulated proximal promoter sequence-specific DNA binding, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, B-lymphocyte, transcription from bacterial-type RNA polymerase promoter, metal ion regulated sequence-specific DNA binding, metal ion regulated proximal promoter sequence-specific DNA binding, TRANSCRIPTION FACTOR, Heterologous, RNA polymerase II distal enhancer sequence-specific bindingfalseTranscription factor Ascl2 promotes geminal center B cell responses through directly regulating AID transcriptionTranscription factor Ascl2 promotes geminal center B cell responses through directly regulating AID transcription2025-09-242021-03-30PRJNA716539GSE16941610090