{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR142/082/SRR14276382/SRR14276382.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR142/084/SRR14276384/SRR14276384.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR142/080/SRR14276380/SRR14276380.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR142/078/SRR14276378/SRR14276378.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR142/085/SRR14276385/SRR14276385.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR142/081/SRR14276381/SRR14276381.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR142/079/SRR14276379/SRR14276379.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR142/083/SRR14276383/SRR14276383.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Guangzhou University of Chinese Medicine"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA723041"],"long_description":["Chromatin immunoprecipitation assay was conducted in rat SNc in sham and model groups. In brief, rat SNc in sham and model groups was gently homogenized to form a single-cell suspension. The suspension was cross-linked for 10 min. Then, after cell lysis, we collected the nuclei of samples. DNA was digested by micrococcal nuclease for 20 min, and the reaction stopped by 0.5 M EDTA. Subsequently, we collected the Nuclear pellet and then incubation in ChIP buffer with protease inhibitors conducted for 10 min on ice. After sonication, the sheared DNA-protein complexes were collected and then were immunoprecipitated with either H3K27Ac or IgG antibody on a rotator for 18 h. Protein G magnetic beads captured DNA-protein complexes. Following capture, DNA fragments were eluted and extracted. The raw reads of samples for rn6 species using ChIP-seq technology service provided by BGI-tech."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"","description":"ChIP-seq with H3K27Ac antibody","dates":{"last_updated":"2023-05-17","first_public":"2021-04-21"},"accession":"PRJNA723041","cross_references":{}}