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Overall design: Routinely cultured MCF7 cells were treated in red phenol-free DMEM medium, supplemented with 2% heat-inactivated and dextran-coated charcoal- treated FBS with estradiol (10-9M), estetrol (10-7M or 10-10M) or vehicle (DMSO 0.001%) for 20h, then progesterone (10-7M) or R5020 (10-8M) were added in some conditions for 4 additional hours. After 24h total treatment, RNA was extracted, quantified and submitted to RNAseq analysis.xref:PubMed:34065180ENASteroid, RNA, RNA Sequence Determination, Analyses, determination, a steroid, Determination, RNA Sequence Determinations, Sequence Determination, Catatoxic Steroids, RNA Sequence, RCB1904, Sequence Determinations, Sequencing, Cell, Determinations, MCF7 cell, MCF7, RNA Sequence Analyses, RNA Sequence Analysis, chemical analysis, RNA Sequencing, Catatoxic., assay, Analysis, steroids, MCF-7 cell, MCF 7 cell, Sequence Analyses, SteroidsSteroid, RNA, RNA Sequence Determination, Analyses, determination, a steroid, Determination, RNA Sequence Determinations, Sequence Determination, Catatoxic Steroids, RNA Sequence, RCB1904, Sequence Determinations, Sequencing, Cell, Determinations, MCF7 cell, MCF7, RNA Sequence Analyses, RNA Sequence Analysis, chemical analysis, RNA Sequencing, Catatoxic., assay, Analysis, steroids, MCF-7 cell, MCF 7 cell, Sequence Analyses, SteroidsfalseRNA sequencing analysis of MCF7 cells treated with several combinations of steroidsRNA sequencing analysis of MCF7 cells treated with several combinations of steroids2025-09-242021-05-20PRJNA725150GSE173300960634065180