{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["UMRS 1138 - Centre de Recherche des Cordeliers"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA732692"],"scientific_name":["Homo sapiens"],"long_description":["The presence of intratumoral tertiary lymphoid structures (TLS) is associated with positive clinical outcomes and responses to immunotherapy in cancer. Here, we used spatial transcriptomics to examine the nature of B cell responses within TLS in renal cell carcinoma (RCC). B cells were enriched in TLS, and therein, we could identify all B cell maturation stages toward plasma cell (PC) formation. B cell repertoire analysis revealed clonal diversification, selection, expansion in TLS, and the presence of fully mature clonotypes at distance. In TLS+ tumors, IgG- and IgA-producing PCs disseminated into the tumor beds along fibroblastic tracks. TLS+ tumors exhibited high frequencies of IgG-producing PCs and IgG-stained and apoptotic malignant cells, suggestive of anti-tumor effector activity. Therapeutic responses and progression-free survival correlated with IgG-stained tumor cells in RCC patients treated with immune checkpoint inhibitors. Thus, intratumoral TLS sustains B cell maturation and antibody production that is associated with response to immunotherapy, potentially via direct anti-tumor effects. Overall design: Spatial transcriptomics of fresh frozen ccRCC human tumors *** Raw data has not been submitted at this time due to patient privacy concerns ***"],"tag":["xref:PubMed:35231421"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Tertiary lymphoid structures generate and propagate anti-tumor antibody-producing plasma cells in renal cell cancer","description":"Tertiary lymphoid structures generate and propagate anti-tumor antibody-producing plasma cells in renal cell cancer","dates":{"last_updated":"2025-09-24","first_public":"2022-03-05"},"accession":"PRJNA732692","cross_references":{"GEO":["GSE175540"],"taxon":["9606"],"PubMed":["35231421"]}}