ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/081/SRR14750881/SRR14750881.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/075/SRR14750875/SRR14750875.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/078/SRR14750878/SRR14750878.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/070/SRR14750870/SRR14750870.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/069/SRR14750869/SRR14750869.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/067/SRR14750867/SRR14750867.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/072/SRR14750872/SRR14750872.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/077/SRR14750877/SRR14750877.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/071/SRR14750871/SRR14750871.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/079/SRR14750879/SRR14750879.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/082/SRR14750882/SRR14750882.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/074/SRR14750874/SRR14750874.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/068/SRR14750868/SRR14750868.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/080/SRR14750880/SRR14750880.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/076/SRR14750876/SRR14750876.fastq.gzprimaryOK200GenomicsMPI Biology of Ageinghttps://www.ebi.ac.uk/ena/browser/view/PRJNA735739Saccharomyces cerevisiaeRibosome biogenesis is an essential cellular process that requires integration of extracellular cues, such as metabolic state, with intracellular signaling, transcriptional regulation and chromatin accessibility at the ribosomal DNA. Here, we demonstrate that the recently identified histone modification, methylation of H2AQ105, is an integral part of a dynamic chromatin network at the rDNA locus. Its deposition depends on a functional mTor signaling pathway as well as acetylation of histone H3 at position K56, thus integrating signals from cell cycle, metabolic and proliferative states. Furthermore, we identify a first epigenetic reader of this modification, the ribonucleoprotein Nhp2, which specifically recognizes the methylation on H2AQ105. Based on functional and proteomic data we suggest that Nhp2 functions as an adapter to bridge the rDNA chromatin with components of the small subunit processome to efficiently coordinate transcription of rRNA with its post-transcriptional processing. We support this by showing that an H2AQ105A mutant has a mild defect in early processing of the rRNA. Overall design: RNA sequencing of four genotypes with 4 replicates each to investigate expression changesxref:PubMed:34409714ENAbiochemical pathways, Metabolic Process, DmelCG5258, degradation, Process, Processes, NOLA2, metabolism resulting in cell growth, Metabolic Concepts, 2410130M07Rik, RNA-seq., Metabolic Processes, Histone, Concept, Metabolic Phenomena, Histone H2b, Metabolism Concepts, Nola2, Histone H2a, Metabolism, DKCB2, Phenomena, Concepts, CG5258, Whole Transcriptome Shotgun Sequencing, secretion, methylation, Metabolism Concept, Phenomenon, Metabolism Phenomena, metabolism, Histone H3.3, Metabolic Phenomenon, multicellular organism metabolic process, biodegradation, Metabolic, catabolism, Metabolic Concept, metabolic process resulting in cell growth, NHP2P, Histone H1(s), single-organism metabolic process, Histone H5, Histone H4, Histone H7, biotransformation, ribosome biogenesis and assembly, Catabolism, Methylations, FBgn0029148, Histone H1, Histone H3, D11Ertd175e, Anabolismbiochemical pathways, Metabolic Process, DmelCG5258, degradation, Process, Processes, NOLA2, metabolism resulting in cell growth, Metabolic Concepts, 2410130M07Rik, RNA-seq., Metabolic Processes, Histone, Concept, Metabolic Phenomena, Histone H2b, Metabolism Concepts, Nola2, Histone H2a, Metabolism, DKCB2, Phenomena, Concepts, CG5258, Whole Transcriptome Shotgun Sequencing, secretion, methylation, Metabolism Concept, Phenomenon, Metabolism Phenomena, metabolism, Histone H3.3, Metabolic Phenomenon, multicellular organism metabolic process, biodegradation, Metabolic, catabolism, Metabolic Concept, metabolic process resulting in cell growth, NHP2P, Histone H1(s), single-organism metabolic process, Histone H5, Histone H4, Histone H7, biotransformation, ribosome biogenesis and assembly, Catabolism, Methylations, FBgn0029148, Histone H1, Histone H3, D11Ertd175e, AnabolismfalseMethylation of Q105 on histone H2A is part of a dynamic regulatory mechanism integrating metabolism with ribosome biogenesis through recruitment of Nhp2 [RNA-Seq]Methylation of Q105 on histone H2A is part of a dynamic regulatory mechanism integrating metabolism with ribosome biogenesis through recruitment of Nhp2 [RNA-Seq]2025-09-242021-06-09PRJNA735739GSE176296493234409714