{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/095/SRR14750595/SRR14750595.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/099/SRR14750599/SRR14750599.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/098/SRR14750598/SRR14750598.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/094/SRR14750594/SRR14750594.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/000/SRR14750600/SRR14750600.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/001/SRR14750601/SRR14750601.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/096/SRR14750596/SRR14750596.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/097/SRR14750597/SRR14750597.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["MPI Biology of Ageing"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA735742"],"scientific_name":["Saccharomyces cerevisiae"],"tag":["xref:PubMed:34409714"],"long_description":["Ribosome biogenesis is an essential cellular process that requires integration of extracellular cues, such as metabolic state, with intracellular signaling, transcriptional regulation and chromatin accessibility at the ribosomal DNA. Here, we demonstrate that the recently identified histone modification, methylation of H2AQ105, is an integral part of a dynamic chromatin network at the rDNA locus. Its deposition depends on a functional mTor signaling pathway as well as acetylation of histone H3 at position K56, thus integrating signals from cell cycle, metabolic and proliferative states. Furthermore, we identify a first epigenetic reader of this modification, the ribonucleoprotein Nhp2, which specifically recognizes the methylation on H2AQ105. Based on functional and proteomic data we suggest that Nhp2 functions as an adapter to bridge the rDNA chromatin with components of the small subunit processome to efficiently coordinate transcription of rRNA with its post-transcriptional processing. We support this by showing that an H2AQ105A mutant has a mild defect in early processing of the rRNA. Overall design: Examination of Nhp2 binding to the DNA. For each genotype, two biological replicates were used. ChIP was performed using Nhp2 antibody. Input samples were taken before addition the antibody as reference."],"repository":["ENA"],"name_synonyms":["biochemical pathways, HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Metabolic Process, ChIP-Chip, DmelCG5258, Chromatin Immuno-precipitation, degradation, Process, Processes, NOLA2, metabolism resulting in cell growth, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Metabolic Concepts, 2410130M07Rik, ChIP Sequencing, CLIP-Seq, Assay for Transposase-Accessible Chromatin Using Sequencing, Metabolic Processes, ChIP-PET, Histone, ChIP-Exo, Concept, Metabolic Phenomena, Histone H2b, Metabolism Concepts, Nola2, Histone H2a, Metabolism, DKCB2, Phenomena, Chromatin Immunoprecipitation Sequencing-Chip, Concepts, CG5258, secretion, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, methylation, Metabolism Concept, Phenomenon, Metabolism Phenomena, metabolism, Histone H3.3, Metabolic Phenomenon, Chromatin Immunoprecipitation Sequencing Chip, multicellular organism metabolic process, Chromatin Immuno precipitation Sequencing, biodegradation, Metabolic, catabolism, ChIP, Chromatin Immunoprecipitation Paired End Tag, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, Metabolic Concept, Chromatin Immunoprecipitation, metabolic process resulting in cell growth, Chromatin Immuno-precipitation Sequencing, ChIP Exonuclease, NHP2P, ChIP-Seq, Histone H1(s), Sequencing, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, single-organism metabolic process, ChIA-PET., Chromatin Immuno-Precipitation Paired-End Tag, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Histone H5, Histone H4, Histone H7, biotransformation, ribosome biogenesis and assembly, Catabolism, ChIP-Exonuclease, Methylations, FBgn0029148, Histone H1, Histone H3, D11Ertd175e, Anabolism"],"description_synonyms":["biochemical pathways, HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, Metabolic Process, ChIP-Chip, DmelCG5258, Chromatin Immuno-precipitation, degradation, Process, Processes, NOLA2, metabolism resulting in cell growth, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, Metabolic Concepts, 2410130M07Rik, ChIP Sequencing, CLIP-Seq, Assay for Transposase-Accessible Chromatin Using Sequencing, Metabolic Processes, ChIP-PET, Histone, ChIP-Exo, Concept, Metabolic Phenomena, Histone H2b, Metabolism Concepts, Nola2, Histone H2a, Metabolism, DKCB2, Phenomena, Chromatin Immunoprecipitation Sequencing-Chip, Concepts, CG5258, secretion, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, methylation, Metabolism Concept, Phenomenon, Metabolism Phenomena, metabolism, Histone H3.3, Metabolic Phenomenon, Chromatin Immunoprecipitation Sequencing Chip, multicellular organism metabolic process, Chromatin Immuno precipitation Sequencing, biodegradation, Metabolic, catabolism, ChIP, Chromatin Immunoprecipitation Paired End Tag, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, Metabolic Concept, Chromatin Immunoprecipitation, metabolic process resulting in cell growth, Chromatin Immuno-precipitation Sequencing, ChIP Exonuclease, NHP2P, ChIP-Seq, Histone H1(s), Sequencing, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, single-organism metabolic process, ChIA-PET., Chromatin Immuno-Precipitation Paired-End Tag, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, Histone H5, Histone H4, Histone H7, biotransformation, ribosome biogenesis and assembly, Catabolism, ChIP-Exonuclease, Methylations, FBgn0029148, Histone H1, Histone H3, D11Ertd175e, Anabolism"],"additional_accession":[]},"is_claimable":false,"name":"Methylation of Q105 on histone H2A is part of a dynamic regulatory mechanism integrating metabolism with ribosome biogenesis through recruitment of Nhp2 [ChIP-Seq]","description":"Methylation of Q105 on histone H2A is part of a dynamic regulatory mechanism integrating metabolism with ribosome biogenesis through recruitment of Nhp2 [ChIP-Seq]","dates":{"last_updated":"2025-09-24","first_public":"2021-06-09"},"accession":"PRJNA735742","cross_references":{"GEO":["GSE176301"],"taxon":["4932"],"PubMed":["34409714"]}}