ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/001/SRR14754801/SRR14754801_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/001/SRR14754801/SRR14754801_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/098/SRR14754798/SRR14754798_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/099/SRR14754799/SRR14754799_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/000/SRR14754800/SRR14754800_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/000/SRR14754800/SRR14754800_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/099/SRR14754799/SRR14754799_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR147/098/SRR14754798/SRR14754798_1.fastq.gzprimaryOK200GenomicsCSIChttps://www.ebi.ac.uk/ena/browser/view/PRJNA735891Sinorhizobium melilotixref:EuropePMC:PMC8844928AbcR1/2 tagged at their 5-ends with the MS2 aptamer were used as baits in affinity chromatography with an MS2-MBP fusion protein immobilized on an amylose resin. Wild type sRNAs (without MS2) were used as controls. Co-purified mRNAs were sequenced on an Illumina platform. Mapping of the sequencing reads to the S. meliloti genome revealed massive AbcR1/2 base-pairing to mRNAs encoding transport/metabolic proteins.ENASinorhizobium meliloti (Dangeard 1926) De Lajudie et al. 1994, Rhizobium meliloti, Ensifer meliloti.Polyadenylated Messenger RNA, Rhizobium meliloti, RNA, Poly(A)+ RNA, Non Polyadenylated mRNA, protein_coding_transcript, RNA Sequence Determination, mRNA, Poly(A) Tail, Analyses, Polyadenylated, Determination, MS/MS, RNA Sequence Determinations, MS2, Sequence Determination, Polyadenylated Messenger, RNA Sequence, E430039A18Rik, Non-Polyadenylated, Messenger RNA, messenger RNA, purification, CD156a, Sequence Determinations, Non-Polyadenylated mRNA, Sequencing, CD156, Determinations, isolation and purification, template RNA, RNA Sequence Analyses, isolation, Messenger, Poly(A)+ mRNA, RNA Sequence Analysis, Ensifer meliloti, Sinorhizobium meliloti (Dangeard 1926) De Lajudie et al. 1994, INSDC_feature:mRNA, RNA Sequencing, Polyadenylated RNA, Analysis, Polyadenylated mRNA, Non Polyadenylated, Poly(A) RNA, tandem MS, Maps., Sequence AnalysesfalseSinorhizobium melilotiThe mRNA interactomes of the homologous Sinorhizobium meliloti trans-sRNAs AbcR1 and AbcR2 determined by MS2-affinity purification coupled with RNA sequencing (MAPS).2025-09-242022-01-28PRJNA735891382