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Health Networkhttps://www.ebi.ac.uk/ena/browser/view/PRJNA737991Homo sapiensxref:PubMed:35405523The purpose of this study was to identify miRNAs that were dysregulated after the onset of COVID-19 and thus potentially be used for risk stratification (i.e., mortality). Therefore, we conducted a multi-center, retrospective longitudinal cohort study enrolling 142 patients with laboratory-confirmed SARS-CoV-2 infection who presented to two Canadian hospitals from May 2020 – December 2020 along with a cohort of 27 SARS-CoV-2 patients with mild upper respiratory tract symptoms and 69 SARS-CoV-2-negative patients from the ICU. Blood was biobanked from SARS-CoV-2 positive patients in the emergency department (mild), ward (moderate) or intensive care unit (severe). Assessment of miRNA expression and co-regulatory network generation revealed significant transcriptome dyregulation in pateints with severe COVID-19 that was largely different from SARS-CoV-2 negative patients in the ICU. Overall design: Platelet reduced plasma from either: (1) SARS-CoV-2 negative patients with mild upper respiratory tract symptoms, (2) patients with mild COVID-19, (3) patients with moderate COVID-19, (4) patients with severe COVID-19, and (5) SARS-CoV-2 negative patients from the ICU with upper respiratory tract illnesses was taken from a -80 freezer and thawed on ice. Sample Groups: (1) n=30 SARS-CoV-2 negative patients with mild upper respiratory tract symptoms (2) n=14 patients with mild COVID-19 (3) n=15 patients with moderate COVID-19 (4) n=45 patients with severe COVID-19 (5) n=31 SARS-CoV-2 negative patients from the ICU with upper respiratory tract symptoms. A total of 30uL of thawed plasma was co-incubated with 1:1 volumes of HTG Plasma Lysis buffer (pre-warmed to 50 degrees celsius) and 1/10th v/v proteinase K for three hours at 50 degrees celsius with shaking (200rpm).ENAFresh, COVID19, RNA, Disease, Coronavirus Disease 19, Frozen Plasma, β-CoV, Fresh Frozen Plasma, beta-CoV, stRNA, Blood, COVID-19, Small Temporal RNA, SARS-CoV-2, Virus Infection, COVID-19 Virus Diseases, 2019 novel coronavirus, Primary, 2019 Novel Coronavirus Disease, SARS-CoV-2 Infections, 2019 nCoV Infection, COVID 19 Virus Infection, Plasmas, Virus Disease, Client, 2019 nCoV Disease, whole transcriptome, COVID-19 Virus Disease, 2019-nCoV Infection, Frozen Plasmas, Small, Micro RNA, 2019-nCoV Disease, SARS CoV 2 Infection, Infection, Severe Acute Respiratory Syndrome Coronavirus 2 Infection, Coronavirus, COVID-19 Virus Infections, β-coronavirus, pre-miRNA, 2019-nCoV, COVID 19 Pandemic, plasma., pri miRNA, Plasma, Fresh Frozen Plasmas, Fresh Frozen, miRNAs, portion of blood plasma, Blood Plasma, Small Temporal, β-CoVs, betacoronavirus, Disease 2019, Pandemic, Micro, Temporal RNA, COVID-19 Virus Infection, Coronavirus Disease 2019, portion of plasma, Primary MicroRNA, severe acute respiratory syndrome coronavirus 2, Coronavirus Disease-19, patient, miRNA, Primary miRNA, COVID-19 Pandemics, SARS-CoV-2 Infection, COVID-19 Pandemic, beta-CoVs, INSDC_feature:ncRNA, pri-miRNA, SARS-coronavirus 2, COVID-19 Virus, blood plasm, Patient, Clients, Blood Plasmas, MicroRNA, 2019 Novel Coronavirus Infection, 2019-nCoV Diseases, pre miRNA, COVID 19 Virus Disease, SARS Coronavirus 2 Infection, COVID 19, 2019-nCoV InfectionsFresh, COVID19, RNA, Disease, Coronavirus Disease 19, Frozen Plasma, β-CoV, Fresh Frozen Plasma, beta-CoV, stRNA, Blood, COVID-19, Small Temporal RNA, SARS-CoV-2, Virus Infection, COVID-19 Virus Diseases, 2019 novel coronavirus, Primary, 2019 Novel Coronavirus Disease, SARS-CoV-2 Infections, 2019 nCoV Infection, COVID 19 Virus Infection, Plasmas, Virus Disease, Client, 2019 nCoV Disease, whole transcriptome, COVID-19 Virus Disease, 2019-nCoV Infection, Frozen Plasmas, Small, Micro RNA, 2019-nCoV Disease, SARS CoV 2 Infection, Infection, Severe Acute Respiratory Syndrome Coronavirus 2 Infection, Coronavirus, COVID-19 Virus Infections, β-coronavirus, pre-miRNA, 2019-nCoV, COVID 19 Pandemic, plasma., pri miRNA, Plasma, Fresh Frozen Plasmas, Fresh Frozen, miRNAs, portion of blood plasma, Blood Plasma, Small Temporal, β-CoVs, betacoronavirus, Disease 2019, Pandemic, Micro, Temporal RNA, COVID-19 Virus Infection, Coronavirus Disease 2019, portion of plasma, Primary MicroRNA, severe acute respiratory syndrome coronavirus 2, Coronavirus Disease-19, patient, miRNA, Primary miRNA, COVID-19 Pandemics, SARS-CoV-2 Infection, COVID-19 Pandemic, beta-CoVs, INSDC_feature:ncRNA, pri-miRNA, SARS-coronavirus 2, COVID-19 Virus, blood plasm, Patient, Clients, Blood Plasmas, MicroRNA, 2019 Novel Coronavirus Infection, 2019-nCoV Diseases, pre miRNA, COVID 19 Virus Disease, SARS Coronavirus 2 Infection, COVID 19, 2019-nCoV InfectionsfalseWhole transcriptome miRNA profiling of Coronavirus Disease 2019 (COVID-19) patient plasma.Whole transcriptome miRNA profiling of Coronavirus Disease 2019 (COVID-19) patient plasma.2025-09-242022-04-20PRJNA737991GSE178246960635405523