ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/030/SRR15632830/SRR15632830.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/041/SRR15632841/SRR15632841.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/036/SRR15632836/SRR15632836.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/025/SRR27980825/SRR27980825_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/042/SRR15632842/SRR15632842.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/029/SRR15632829/SRR15632829.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/035/SRR15632835/SRR15632835.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/031/SRR15632831/SRR15632831.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/033/SRR15632833/SRR15632833.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/040/SRR15632840/SRR15632840.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/034/SRR15632834/SRR15632834.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/038/SRR15632838/SRR15632838_subreads.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR280/075/SRR28000275/SRR28000275_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/037/SRR15632837/SRR15632837.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/039/SRR15632839/SRR15632839.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR280/076/SRR28000276/SRR28000276_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR279/024/SRR27980824/SRR27980824_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/028/SRR15632828/SRR15632828.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR156/032/SRR15632832/SRR15632832.fastq.gzprimaryOK200Genomicssalim bougouffahttps://www.ebi.ac.uk/ena/browser/view/PRJNA743275Eucalyptus camaldulensisCurrently, different sequencing platforms are used to generate plant genomes. Presently, no workflow has been properly developed to optimize time, cost, and assembly quality. We present LeafGo, a complete de novo plant genome workflow, that starts from tissue and produces genomes with modest laboratory and bioinformatic resources in seven days and using one long-read sequencing technology. LeafGo was optimized with ten different plant species, three of which were used to generate high-quality chromosome level assemblies without any scaffolding technologies. We report the diploid genomes of Eucalyptus rudis and E. camaldulensis and the allotetraploid genome of Arachis hypogaea.ENAfalseEucalyptus camaldulensis Umbrella project2025-09-242021-07-04PRJNA74327534316