{"database":"ENA","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["4503, Jeffrey Cheah School of Medicine and Health Sciences, Monash university Malaysia"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA743282"],"scientific_name":["Homo sapiens"],"long_description":["Diarylpentanoids exhibit a high degree of anti-cancer activity and stability in vitro over curcumin in prostate cancer cells. Hence, this study aims to investigate the effects of a diarylpentanoid, 1,5-bis(4-hydroxy-3-methoxyphenyl)-1,4-pentadiene-3-one (MS13) on cytotoxicity, anti-proliferative, apoptosis-inducing, anti-migration properties, and the underlying molecular mechanisms on treated androgen-independent prostate cancer cells, DU 145 and PC-3. A cell viability assay has shown greater cytotoxicity effects of MS13-treated DU 145 cells (EC50 7.57 ± 0.2 µM) and PC-3 cells (EC50 7.80 ± 0.7 µM) compared to curcumin (EC50: DU 145 34.25 ± 2.7 µM and PC-3 27.77± 6.4 µM). In addition, MS13 exhibited significant anti-proliferative activity against AIPC cells compared to curcumin in a dose- and time-dependent manner. Morphological observation, increased caspase-3 activity, and reduced Bcl-2 protein levels in these cells indicated that MS13 induces apoptosis in a time- and dose-dependent. Moreover, MS13 effectively inhibited the migration of DU 145 and PC-3 cells. Our results suggest that cell cycle-apoptosis and PI3K pathways were the topmost significant pathways impacted by MS13 activity. Our findings suggest that MS13 may demonstrate the anti-cancer activity by modulating DEGs associated with the cell cycle-apoptosis and PI3K pathways, thus inhibiting cell proliferation and cell migration and inducing apoptosis in AIPC cells. Overall design: The experiments were performed with three controls and three treated samples for each cell lines, DU 145 and PC-3. Three biological replicates were analyzed and three technical repeats were performed per sample."],"tag":["xref:PubMed:34366863"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Anti-proliferative, Apoptosis and Anti-migration Properties on Androgen-independent Human Prostate Cancer by a Diarylpentanoid, MS13.","description":"Anti-proliferative, Apoptosis and Anti-migration Properties on Androgen-independent Human Prostate Cancer by a Diarylpentanoid, MS13.","dates":{"last_updated":"2025-09-24","first_public":"2021-07-09"},"accession":"PRJNA743282","cross_references":{"GEO":["GSE179324"],"taxon":["9606"],"PubMed":["34366863"]}}