ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR153/054/SRR15341754/SRR15341754.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR153/050/SRR15341750/SRR15341750.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR153/055/SRR15341755/SRR15341755.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR153/053/SRR15341753/SRR15341753.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR153/051/SRR15341751/SRR15341751.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR153/048/SRR15341748/SRR15341748.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR153/049/SRR15341749/SRR15341749.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR153/052/SRR15341752/SRR15341752.fastq.gzprimary200OKGenomicsTakaomi Sanda Lab, Cancer Science Institute of Singapore, National University of Singaporehttps://www.ebi.ac.uk/ena/browser/view/PRJNA752041Homo sapiensRNA-seq analysis was performed in two T-cell acute lymphoblastic leukemia (T-ALL) cell lines (Jurkat and DND-41) to analyze gene expression changes after the treatment with PIK-75. Overall design: Two T-ALL cell lines (Jurkat and DND-41) were treated with DMSO (control) or PIK-75 (120nM) for 4 hours. Synthesized RNAs (spike-in) was added to the samples based on the cell number. Total RNA was extracted using QIAzol lysis reagent, miRNeasy Mini Kit, and DNase I (Qiagen). Strand-specific library construction and 100-bp paired-end sequencing with a sequencing depth of 20 M reads on the DNBSEQ platform were performed at BGI Genomics.xref:PubMed:36073513ENAfalseRNA-seq analysis after PIK75 treatment in T-ALL cellsRNA-seq analysis after PIK75 treatment in T-ALL cells2025-09-242022-10-07PRJNA752041GSE181435960636073513