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Cancer Research Center, Columbia University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA752785"],"scientific_name":["Homo sapiens"],"tag":["xref:PubMed:35440621"],"long_description":["Tissue-specific transcriptional activity is silenced in mitotic cells but it remains unclear whether the mitotic regulatory machinery interacts with tissue-specific transcriptional programs. We show that such cross-talk involves the controlled interaction between core subunits of the anaphase-promoting complex (APC) and the ID2 substrate. The N-terminal region of ID2 bound to core APC subunits, which formed an ID2-compatible pocket that optimally oriented ID2 in the APC-CDH1 complex. Phosphorylation of serine-5 by CDK1 prevented the association of ID2 with core APC, impaired ubiquitylation and stabilized ID2 protein at the mitosis-G1 transition leading to inhibition of basic Helix-Loop-Helix (bHLH)-mediated transcription. The serine-5 phosphomimetic mutant of ID2 that inefficiently bound core APC remained stable during mitosis, delayed exit from mitosis and reloading of bHLH transcription factors on chromatin. It also locked cells into a “mitotic stem cell” transcriptional state resembling the pluripotent program of embryonic stem cells. The substrates of APC-CDH1 SKP2 and Cyclin B1 share with ID2 the phosphorylation-dependent, D-box-independent interaction with core APC. These results reveal a new layer of control of the mechanism by which substrates are recognized by APC. Overall design: RNA-Seq profiling of 27 samples (3 cell conditions: ID2 S5D, ID2 WT, EV 3 time points: 0 h, 8 h, 10 h 3 replicates each)."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Regulated interaction of ID2 with the anaphase-promoting complex links progression through mitosis with reactivation of cell-type-specific transcription","description":"Regulated interaction of ID2 with the anaphase-promoting complex links progression through mitosis with reactivation of cell-type-specific transcription","dates":{"last_updated":"2025-09-24","first_public":"2022-03-20"},"accession":"PRJNA752785","cross_references":{"GEO":["GSE181639"],"taxon":["9606"],"PubMed":["35440621"]}}