{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/042/SRR15532642/SRR15532642_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/040/SRR15532640/SRR15532640_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/044/SRR15532644/SRR15532644_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/046/SRR15532646/SRR15532646_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/038/SRR15532638/SRR15532638_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/042/SRR15532642/SRR15532642_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/041/SRR15532641/SRR15532641_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/044/SRR15532644/SRR15532644_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/045/SRR15532645/SRR15532645_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/038/SRR15532638/SRR15532638_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/041/SRR15532641/SRR15532641_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/043/SRR15532643/SRR15532643_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/047/SRR15532647/SRR15532647_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/047/SRR15532647/SRR15532647_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/039/SRR15532639/SRR15532639_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/043/SRR15532643/SRR15532643_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/045/SRR15532645/SRR15532645_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/046/SRR15532646/SRR15532646_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/039/SRR15532639/SRR15532639_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR155/040/SRR15532640/SRR15532640_2.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Molecular Physiology & Biophysics, University of Iowa"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA756362"],"scientific_name":["Nakaseomyces glabratus CBS 138"],"long_description":["To determine the genomic binding sites for the Candida glabrata transcription factor Upc2A, we utilized three different strains. One was the wild-type KKY2001 which contains a wild-type copy of UPC2A but lacks any HA tag. The other two are both derived from KKY2001 but contain a 3X HA tag immediately after the start codon of UPC2A. These strains are BVGC82 and BVGC84, respectively. Both contain a single copy of a loxP element located 252 bp downstream from the UPC2A stop codon. BVGC82 contains a wild-type copy of the UPC2A gene while BVGC84 contains a mutant form of UPC2A (G898D) that confers elevated resistance to fluconazole. ChIP was performed with anti-HA antibodies in all cases. Strains containing the wild-type UPC2A gene (no HA tag) are referred to as C1 and C2. Strains containing the 3X HA epitope tag at the N-terminus of the wild-type UPC2A gene are referred to as wt1 and wt2. These same strains challenged with fluconazole prior to ChIP are referred to as wtf1 and wtf2. The strain containing the G898D UPC2A allele are referred to as M1 and M2 (no fluconazole challenge) and MF1 and MF2 (with fluconazole challenge. Overall design: ChIP-seq using anti-HA antibody against strains expressing HA-tagged Upc2A as a wild-type protein or a gain-of-function form (G899D Upc2A). Prior to chromatin preparation and shearing, parallel cultures were either left untreated or challenged with 20 microgram/ml of fluconazole for two hours at 30 degrees C."],"tag":["pathogen:priority","pathogen","xref:PubMed:34591857","pathogen:fungus"],"repository":["ENA"],"classification":["fungi"],"description_synonyms":["HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, RNA polymerase II core promoter proximal region sequence-specific binding, T-cell leukemia, ChIP-Chip, Chromatin Immuno-precipitation, determination, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, AGL4, ChIP Sequencing, CLIP-Seq, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, Factor, Assay for Transposase-Accessible Chromatin Using Sequencing, SEPALLATA 2, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, ChIP-PET, Transcription Factor, ChIP-Exo, transcription factor activity, zinc ion regulated core promoter proximal region sequence-specific DNA binding, Candida glabrata, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, chemical analysis, Chromatin Immunoprecipitation Sequencing-Chip, RNA polymerase II transcription factor activity, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, KNOTTED1-like homeobox gene 5, ChIA-PET, F14P3.4, Chromatin Immunoprecipitation Sequencing Chip, Transcription, Chromatin Immuno precipitation Sequencing, Factors, Genomes, ChIP, Chromatin Immunoprecipitation Paired End Tag, ligand, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, F14P3_4, F10N7_150, Chromatin Immunoprecipitation, Chromatin Immuno-precipitation Sequencing, whole genome, ChIP Exonuclease, metal ion regulated core promoter proximal region sequence-specific binding, copper ion regulated core promoter proximal region sequence-specific binding, Cryptococcus glabratus, ChIP-Seq, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, Transcription factor, Sequencing, F10N7.150, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, RNA polymerase II proximal promoter sequence-specific DNA binding, ChIA-PET., Chromatin Immuno-Precipitation Paired-End Tag, copper ion regulated proximal promoter sequence-specific DNA binding, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, AGAMOUS-like 4, Torulopsis glabrata, zinc ion regulated proximal promoter sequence-specific DNA binding, assay, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, ChIP-Exonuclease, metal ion regulated sequence-specific DNA binding, metal ion regulated proximal promoter sequence-specific DNA binding, TRANSCRIPTION FACTOR, RNA polymerase II distal enhancer sequence-specific binding"],"name_synonyms":["HITS-CLIP, High Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, RNA polymerase II core promoter proximal region sequence-specific binding, T-cell leukemia, ChIP-Chip, Chromatin Immuno-precipitation, determination, Cross Linking and Immunoprecipitation Followed by Deep Sequencing, AGL4, ChIP Sequencing, CLIP-Seq, zinc ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, homeobox 1, Factor, Assay for Transposase-Accessible Chromatin Using Sequencing, SEPALLATA 2, metal ion regulated sequence-specific DNA binding RNA polymerase II transcription factor activity, ChIP-PET, Transcription Factor, ChIP-Exo, transcription factor activity, zinc ion regulated core promoter proximal region sequence-specific DNA binding, Candida glabrata, RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity, chemical analysis, Chromatin Immunoprecipitation Sequencing-Chip, RNA polymerase II transcription factor activity, High-Throughput Sequencing of RNA Isolated by Crosslinking Immunoprecipitation, sequence-specific distal enhancer binding RNA polymerase II transcription factor activity, metal ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, KNOTTED1-like homeobox gene 5, ChIA-PET, F14P3.4, Chromatin Immunoprecipitation Sequencing Chip, Transcription, Chromatin Immuno precipitation Sequencing, Factors, Genomes, ChIP, Chromatin Immunoprecipitation Paired End Tag, ligand, Chromatin Immuno Precipitation Paired End Tag, Cross-Linking and Immunoprecipitation Followed by Deep Sequencing, F14P3_4, F10N7_150, Chromatin Immunoprecipitation, Chromatin Immuno-precipitation Sequencing, whole genome, ChIP Exonuclease, metal ion regulated core promoter proximal region sequence-specific binding, copper ion regulated core promoter proximal region sequence-specific binding, Cryptococcus glabratus, ChIP-Seq, RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, Transcription factor, Sequencing, F10N7.150, Assay for Transposase Accessible Chromatin Using Sequencing, Chromatin Immunoprecipitation Paired-End Tag, RNA polymerase II proximal promoter sequence-specific DNA binding, ChIA-PET., Chromatin Immuno-Precipitation Paired-End Tag, copper ion regulated proximal promoter sequence-specific DNA binding, ATAC-Seq, Chromatin Immunoprecipitation Sequencing-Chips, copper ion regulated core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, AGAMOUS-like 4, Torulopsis glabrata, zinc ion regulated proximal promoter sequence-specific DNA binding, assay, sequence-specific transcription regulatory region DNA binding RNA polymerase II transcription factor recruiting transcription factor activity, ChIP-Exonuclease, metal ion regulated sequence-specific DNA binding, metal ion regulated proximal promoter sequence-specific DNA binding, TRANSCRIPTION FACTOR, RNA polymerase II distal enhancer sequence-specific binding"],"additional_accession":[]},"is_claimable":false,"name":"ChIP-seq analysis of transcription factor Upc2A binding across the Candida glabrata genome [Upc2A ChIP-seq]","description":"ChIP-seq analysis of transcription factor Upc2A binding across the Candida glabrata genome [Upc2A ChIP-seq]","dates":{"last_updated":"2025-09-24","first_public":"2021-09-26"},"accession":"PRJNA756362","cross_references":{"GEO":["GSE182488"],"taxon":["284593"],"PubMed":["34591857"]}}