ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR159/015/SRR15901615/SRR15901615_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR159/016/SRR15901616/SRR15901616_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR159/015/SRR15901615/SRR15901615_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR159/014/SRR15901614/SRR15901614_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR159/014/SRR15901614/SRR15901614_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR159/016/SRR15901616/SRR15901616_1.fastq.gzprimary200OKGenomicsTianjin Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA763329Here, Corynebacterium glutamicum was systematically engineered for rapid non-natural xylose metabolism and the underlying adaptations were elucidated by combining metabolic engineering, adaptive laboratory evolution and systems biology techniques. A plasmid-free, stable and efficient xylose-utilizing chassis strain, named CGS15, was reconstructed with both a rapid specific growth rate of 0.341 h-1 on xylose and an excellent co-utilization of glucose and xylose at a ratio of about 2:1.xref:EuropePMC:PMC9746319ENAfalseGlobal cellular metabolic rewiring adapts Corynebacterium glutamicum to efficient non-natural substrate utilization2023-05-172022-10-07PRJNA763329