ENAGenomicsToxicogenomics Lab, CFSAN/OARSA/DT, USFDAhttps://www.ebi.ac.uk/ena/browser/view/PRJNA777268Homo sapiensxref:PubMed:35371477xref:PubMed:35898856We devised two hepatocyte differentiation methods, namely Method 1 (M1) and Method 2 (M2), through modifying existing well-known hepatocyte differentiation strategies, and compared the resultant cells phenotypically, functionally and transcriptomically at different stages of hepatocyte differentiation. !Series_summary =We used microarrays to compare the global gene expression of the HLCs differentiated using the two methods, M1 and M2. Overall design: HLCs were collected at day 17 (D17) and day 21 (D21) for RNA extraction and hybridization on Affymetrix microarrays. Primary human hepatocytes (PHHs) were also included in the study for comparison.ENAHuman Induced Pluripotent Stem Cells., distinct from, Fibroblast-Derived IPS Cells, human being, Man (Taxonomy), Fibroblast-Derived IPS Cell, parenchymal hepatic cell, liver parenchymal cell, Induced Pluripotent Stem Cell, IPS, Modern, Fibroblast-Derived IPS, Hepatocyte, Fibroblast Derived IPS Cells, hiPSC, Fibroblast-Derived, IPS Cells, Cell, human, 410I21.SP6, Human, Hepatic Cells, D16Jhu34, Homo sapiens, Fibroblast Derived Induced Pluripotent Stem Cells, Hepatic Cell, Modern Man, Cells, Fibroblast-Derived Induced Pluripotent Stem Cells, Hepatic, parenchymal liver cell, HCS, Human Induced Pluripotent Stem Cell, Man, IPS CellHuman Induced Pluripotent Stem Cells., distinct from, Fibroblast-Derived IPS Cells, human being, Man (Taxonomy), Fibroblast-Derived IPS Cell, parenchymal hepatic cell, liver parenchymal cell, Induced Pluripotent Stem Cell, IPS, Modern, Fibroblast-Derived IPS, Hepatocyte, Fibroblast Derived IPS Cells, hiPSC, Fibroblast-Derived, IPS Cells, Cell, human, 410I21.SP6, Human, Hepatic Cells, D16Jhu34, Homo sapiens, Fibroblast Derived Induced Pluripotent Stem Cells, Hepatic Cell, Modern Man, Cells, Fibroblast-Derived Induced Pluripotent Stem Cells, Hepatic, parenchymal liver cell, HCS, Human Induced Pluripotent Stem Cell, Man, IPS CellfalseExpression data from hepatocyte-like cells (HLCs) differentiated from human induced pluripotent stem cells (iPSCs)Expression data from hepatocyte-like cells (HLCs) differentiated from human induced pluripotent stem cells (iPSCs)2025-09-242021-11-05PRJNA777268GSE18701196063589885635371477