ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/002/SRR17256402/SRR17256402.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/013/SRR17256413/SRR17256413.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/007/SRR17256407/SRR17256407.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/005/SRR17256405/SRR17256405.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/011/SRR17256411/SRR17256411.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/010/SRR17256410/SRR17256410.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/004/SRR17256404/SRR17256404.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/000/SRR17256400/SRR17256400.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/098/SRR17256398/SRR17256398.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/009/SRR17256409/SRR17256409.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/006/SRR17256406/SRR17256406.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/012/SRR17256412/SRR17256412.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/008/SRR17256408/SRR17256408.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/099/SRR17256399/SRR17256399.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/001/SRR17256401/SRR17256401.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR172/003/SRR17256403/SRR17256403.fastq.gzprimaryOK200GenomicsHippenmeyer, Institute of Science and Technology Austria (IST Austria)https://www.ebi.ac.uk/ena/browser/view/PRJNA789773Mus musculusThe generation of a correctly-sized cerebral cortex with all-embracing neuronal and glial cell-type diversity critically depends on faithful radial glial progenitor (RGP) cell proliferation/differentiation programs. Temporal RGP lineage progression is regulated by Polycomb Repressive Complex 2 (PRC2) and loss of PRC2 activity results in severe neurogenesis defects and microcephaly. How PRC2-dependent gene expression instructs RGP lineage progression is unknown. Here we utilize Mosaic Analysis with Double Markers (MADM)-based single cell technology and demonstrate that PRC2 is not cell-autonomously required in neurogenic RGPs but rather acts at the global tissue-wide level. Conversely, cortical astrocyte production and maturation is cell-autonomously controlled by PRC2-dependent transcriptional regulation. We thus reveal highly distinct and sequential PRC2 functions in RGP lineage progression that are dependent on complex interplays between intrinsic and tissue-wide mechanisms. In a broader context our results imply a critical role for the genetic and cellular niche environment in neural stem cell behavior. Overall design: RNA-Seq of enriched astrocytes from control and sparse deletion of Eed in mouse neocortex using a MADM systemxref:PubMed:36322669ENAfalseTissue-wide Genetic and Cellular Landscape Instructs the Execution of Sequential PRC2 Functions in Neural Stem Cell Lineage Progression [astro]Tissue-wide Genetic and Cellular Landscape Instructs the Execution of Sequential PRC2 Functions in Neural Stem Cell Lineage Progression [astro]2025-09-242022-10-07PRJNA789773GSE1911071009036322669