{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/057/SRR17330957/SRR17330957_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/056/SRR17330956/SRR17330956_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/055/SRR17330955/SRR17330955_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/054/SRR17330954/SRR17330954_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/056/SRR17330956/SRR17330956_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/055/SRR17330955/SRR17330955_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/057/SRR17330957/SRR17330957_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/054/SRR17330954/SRR17330954_1.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["School of Life Sciences"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA792207"],"long_description":["RNA degradosome plays key roles in pre-rRNA maturation and mRNA decay, and mediates isoniazid tolerance in Mycobacterium tuberculosis (Mtb). Mtb ribonuclease J (Mtb-RNase J) is one core enzyme of the RNA degradosome, which comprises RNases, RNA helicases, and glycolytic enzymes. However, how Mtb-RNase J functions in RNA metabolism keeps unclear. Here, we solved the crystal structures of Mtb-RNase J and its complex with single-strand RNA (ssRNA). RNA binding induced the comformation change. Mtb-RNase J showed typical beta lactamase activity and exo/endoribonuclease activities. Mn2+ could dramatically enhanced the ribonuclease activity of Mtb-RNase J, which prefers to bind 8-9 nt ssRNA.We unveiled the novel mechanism of Mtb-RNase J to form the active ribonuclease homodimer. Knockout of RNase J dramatically changed the expression levels of 49 genes in the metabolic pathways and morphologies of Mycobacterium smegmatis. Thus, current study explored the structural basis and function of Mtb-RNase J in RNA metabolism."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"","description":"Structure insight of ribonuclease J reveals the basis of RNA metabolism in Mycobacterium tuberculosis","dates":{"last_updated":"2023-05-19","first_public":"2023-01-08"},"accession":"PRJNA792207","cross_references":{}}