{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/016/SRR17359216/SRR17359216_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/014/SRR17359214/SRR17359214_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR222/028/SRR22255928/SRR22255928_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR222/029/SRR22255929/SRR22255929_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/012/SRR17359212/SRR17359212_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/013/SRR17359213/SRR17359213_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/017/SRR17359217/SRR17359217_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/014/SRR17359214/SRR17359214_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR222/029/SRR22255929/SRR22255929_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/015/SRR17359215/SRR17359215_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/013/SRR17359213/SRR17359213_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/017/SRR17359217/SRR17359217_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/016/SRR17359216/SRR17359216_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR222/028/SRR22255928/SRR22255928_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/012/SRR17359212/SRR17359212_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR173/015/SRR17359215/SRR17359215_2.fastq.gz"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Institute of Human Virology, Zhongshan School of Medicine, Sun Yat-Sen University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA792846"],"scientific_name":["Homo sapiens"],"long_description":["To investigate the regulation of ER stress-related gene expression by KSHV-ORF45 during lytic replication, we performed RNA-sequencing analysis of iSLK-BAC16 vs. iSLK-STOP45 cells under lytic induction for 72h. When the differentially expressed genes were filtered and analyzed, we found that ER stress-related gene expression was much low in iSLK-STOP45 cells compared with iSLK-BAC16 cells, indicating that ORF45 expression is required for induction of ER stress.To further reveal the signal transduction of LAMP3 in KSHV lytic replication, RNA-sequencing analysis was performed to identify the differentially expressed genes (DEGs) in normal vs. LAMP3-silenced cells under lytic replication. A total of 35372 raw read targets were obtained and all DEG clusters were filtered and analyzed by KEGG pathway enrichment analysis. Eleven pathway were significantly enriched over 10 fold in LAMP3-depleted cells compared with control cells, including PI3K-Akt signaling pathway.Given that Akt and ERK activation play the important roles in KSHV lytic replication, we conclude that LAMP3 might promote Akt and ERK activation and then consequently facilitate KSHV lytic replication. Overall design: mRNA profiles of iSLK-BAC16 vs. iSLK-STOP45 cells under lytic induction for 72h"],"tag":["xref:PubMed:36377873"],"repository":["ENA"],"name_synonyms":["Connective Tissue Sarcoma, Malignant Soft Tissue Tumor, MAL NEO SOFT TISSUE LEG, sarcoma, Sarcoma of the Soft Tissue and Bone, Mesenchymal tumor, CREB-2, DCLAMP, axis, MAL NEO SOFT TISSUE NEC, malignant, Atf-4, Spindle Cell Sarcomas, Malignant neoplasm of connective and other soft tissue of lower limb, Up-Regulation (Physiology), TSC403, stalk, LAMP-3, Malignant neoplasm of connective and other soft tissue of thorax, Malignant neoplasm of other specified sites of connective and other soft tissue, [M]Sarcoma NOS, TXREB, site unspecified, Sarcoma of the Soft Tissue, Malignant neoplasm of connective and other soft tissue, unspecified, dATF-4, Soft Tissue Sarcoma, Sarcoma of Soft Tissue, including shoulder, Epithelioid Sarcomas, Malignant tumour of soft tissue, MAL NEO SOFT TISSUE HEAD, Soft tissue tumour, Malignant neoplasm of soft tissue, CREB2, [M]Sarcoma NOS (morphologic abnormality), NOS, l(2)crc, tumour of soft tissue and skeleton, Malignant mesenchymal tumour, Sarcoma of Soft Tissue and Bone, MAL NEO SOFT TIS PELVIS, DC LAMP, Atf4, Epithelioid, 929, DmelCG8669, Malignant neoplasm of connective and other soft tissue of upper limb, Sarcomas, Malignant Tumor of the Soft Tissue, MAL NEO SOFT TIS ABDOMEN, Malignant neoplasm of connective and other soft tissue (disorder), Malignant neoplasm of connective and other soft tissue of head, MAL NEOPL TRUNK NOS., Cd208, Spindle Cell, MAL NEO SOFT TISSUE NOS, Upregulation, Receptor Up-Regulation, including hip, Up Regulation, C2, MAL NEO SOFT TIS THORAX, and neck, C|ATF, Soft Tissue Sarcomas, Malignant neoplasm of connective and other soft tissue of abdomen, Malignant neoplasm of connective and other soft tissue of trunk, Ire1, tumor of soft tissue and skeleton, Malignant Soft Tissue Neoplasm, culm, ATF-4, TAXREB67, Sarcoma, no ICD-O subtype, Malignant neoplasm of soft tissue (disorder), no ICD-O subtype (morphologic abnormality), Epithelioid Sarcoma, Malignant tumor of soft tissue, Malignant Neoplasm of the Soft Tissue, CG8669, face, 1200002D17Rik, Malignant neoplasm of connective and other soft tissue of pelvis, CD208, Spindle Cell Sarcoma, ATF4/crc, LAMP, DC-LAMP, Soft Tissue, connective and soft tissue neoplasm, MAL NEO SOFT TISSUE ARM"],"description_synonyms":["Connective Tissue Sarcoma, Malignant Soft Tissue Tumor, MAL NEO SOFT TISSUE LEG, sarcoma, Sarcoma of the Soft Tissue and Bone, Mesenchymal tumor, CREB-2, DCLAMP, axis, MAL NEO SOFT TISSUE NEC, malignant, Atf-4, Spindle Cell Sarcomas, Malignant neoplasm of connective and other soft tissue of lower limb, Up-Regulation (Physiology), TSC403, stalk, LAMP-3, Malignant neoplasm of connective and other soft tissue of thorax, Malignant neoplasm of other specified sites of connective and other soft tissue, [M]Sarcoma NOS, TXREB, site unspecified, Sarcoma of the Soft Tissue, Malignant neoplasm of connective and other soft tissue, unspecified, dATF-4, Soft Tissue Sarcoma, Sarcoma of Soft Tissue, including shoulder, Epithelioid Sarcomas, Malignant tumour of soft tissue, MAL NEO SOFT TISSUE HEAD, Soft tissue tumour, Malignant neoplasm of soft tissue, CREB2, [M]Sarcoma NOS (morphologic abnormality), NOS, l(2)crc, tumour of soft tissue and skeleton, Malignant mesenchymal tumour, Sarcoma of Soft Tissue and Bone, MAL NEO SOFT TIS PELVIS, DC LAMP, Atf4, Epithelioid, 929, DmelCG8669, Malignant neoplasm of connective and other soft tissue of upper limb, Sarcomas, Malignant Tumor of the Soft Tissue, MAL NEO SOFT TIS ABDOMEN, Malignant neoplasm of connective and other soft tissue (disorder), Malignant neoplasm of connective and other soft tissue of head, MAL NEOPL TRUNK NOS., Cd208, Spindle Cell, MAL NEO SOFT TISSUE NOS, Upregulation, Receptor Up-Regulation, including hip, Up Regulation, C2, MAL NEO SOFT TIS THORAX, and neck, C|ATF, Soft Tissue Sarcomas, Malignant neoplasm of connective and other soft tissue of abdomen, Malignant neoplasm of connective and other soft tissue of trunk, Ire1, tumor of soft tissue and skeleton, Malignant Soft Tissue Neoplasm, culm, ATF-4, TAXREB67, Sarcoma, no ICD-O subtype, Malignant neoplasm of soft tissue (disorder), no ICD-O subtype (morphologic abnormality), Epithelioid Sarcoma, Malignant tumor of soft tissue, Malignant Neoplasm of the Soft Tissue, CG8669, face, 1200002D17Rik, Malignant neoplasm of connective and other soft tissue of pelvis, CD208, Spindle Cell Sarcoma, ATF4/crc, LAMP, DC-LAMP, Soft Tissue, connective and soft tissue neoplasm, MAL NEO SOFT TISSUE ARM"],"additional_accession":[]},"is_claimable":false,"name":"Up-regulation of ATF4-LAMP3 axis by ORF45 facilitate lytic replication of Kaposi’s sarcoma-associated herpesvirus","description":"Up-regulation of ATF4-LAMP3 axis by ORF45 facilitate lytic replication of Kaposi’s sarcoma-associated herpesvirus","dates":{"last_updated":"2025-09-24","first_public":"2022-11-24"},"accession":"PRJNA792846","cross_references":{"GEO":["GSE192724"],"taxon":["9606"],"PubMed":["36377873"]}}