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Total RNA was extracted using the TRIzol reagent (Invitrogen, Waltham, MA, USA). DNase (Promega, USA) was used to remove potential DNA contamination. For the quantitative real-time polymerase chain reaction (qRT-PCR) analysis Results:In this study, there were extensive variations in gene expression patterns of this hybrid, and down-regulated expression of key genes for pollen development, such as meiosis of pollen mother cells, DNA damage repair and pollen sperm cell differentiation, may lead to pollen abortion. Styrene-propyl synthesis and wax biosynthesis pathway were blocked, which affected pollen wall formation. Conclusions:The wide variation of genes showed that the expression of A and B genomes were affected differently. B genome tended to downregulate the expression of key genes during meiosis and mitosis, while A genome tended to downregulate the expression of genes related to stress and pollen wall formation. The results showed that the development of male gametophyte was seriously affected after synthesis. Overall design: mRNA profiles of male gametophytes"],"repository":["ENA"],"description_synonyms":["meiosis, Subfecundity, Pollen Grain, artificial sequence, Plant Male, Male, Phases, Gene, Fertility, Pollen, Male Gametes, Gene Expression Profilings, World Fertility Survey, Transcript Expression Analysis, Fertility Preferences, mRNA Differential Displays, Mitotic M, Determinant, Gene Expression Pattern Analysis, exact), Below Replacement, B830015H24, Gene Expression Monitorings, Trep132, Survey, Preference, Analysis, Profilings, World Fertility, synthetic genetic interaction (sensu inequality), Fertility Surveys, Fecundity, Gametes, Fertility Incentives, Pollen Grains, Plant Microspores, Grain, RAPA, M Phases, Microspores, polen (Spanish, Analyses, Natural, Kale, Gametophytes, synthetic genetic interaction defined by inequality, Transcriptomics, Meioses, Transcript Expression, Transcriptome Analysis, Cauliflower, Cabbage, Trep-132., Monitoring, Gene Expression Analysis, Meiotic, male generative cell, Cabbages, Below Replacement Fertility, TREP132, Plant Male Gamete, Fertility Determinant, Fertility Preference, 花粉 (Japanese, Fertility Incentive, Expression Analyses, Differential Fertility, TReP-132, Plant Microspore, BCAR2, Profiling, Transcriptome, Meiotic M Phase, Male Gametophytes, World, World Fertility Surveys, Monitorings, Brussel Sprout, artificial gene, Gametophyte, synthetic DNA, Collard Greens, Male Gamete, Male Gametophyte, Gene Expression, Differential Display, Transcript Expression Analyses, pollen grain (exact), mitosis, Gamete, Natural Fertility, synthetic, Broccoli, Mitotic M Phase, Determinants, mRNA Differential Display, M Phase, Meiotic M Phases, dJ139D8.5, Gene Expression Monitoring, Phase, Meiotic M, RP1-139D8.5, Transcriptome Profiling, Mitoses, mRNA, Transcriptome Profilings, Plant, Differential Displays, synthetic constructs, Transcriptome Analyses, Preferences, Fertility Determinants, Grains, Differential, SYNTHETIC CONSTRUCT sequences, Plant Male Gametes, Gene Expression Analyses, 9430096I18Rik, Marital, Collard Green, Mitotic M Phases, Surveys, AI429294, artificial, Expression Analysis, Mitotic, Microspore, Fecundability, HSA277276, Fertility Survey, pollen grain, Marital Fertility"],"name_synonyms":["meiosis, Subfecundity, Pollen Grain, artificial sequence, Plant Male, Male, Phases, Gene, Fertility, Pollen, Male Gametes, Gene Expression Profilings, World Fertility Survey, Transcript Expression Analysis, Fertility Preferences, mRNA Differential Displays, Mitotic M, Determinant, Gene Expression Pattern Analysis, exact), Below Replacement, B830015H24, Gene Expression Monitorings, Trep132, Survey, Preference, Analysis, Profilings, World Fertility, synthetic genetic interaction (sensu inequality), Fertility Surveys, Fecundity, Gametes, Fertility Incentives, Pollen Grains, Plant Microspores, Grain, RAPA, M Phases, Microspores, polen (Spanish, Analyses, Natural, Kale, Gametophytes, synthetic genetic interaction defined by inequality, Transcriptomics, Meioses, Transcript Expression, Transcriptome Analysis, Cauliflower, Cabbage, Trep-132., Monitoring, Gene Expression Analysis, Meiotic, male generative cell, Cabbages, Below Replacement Fertility, TREP132, Plant Male Gamete, Fertility Determinant, Fertility Preference, 花粉 (Japanese, Fertility Incentive, Expression Analyses, Differential Fertility, TReP-132, Plant Microspore, BCAR2, Profiling, Transcriptome, Meiotic M Phase, Male Gametophytes, World, World Fertility Surveys, Monitorings, Brussel Sprout, artificial gene, Gametophyte, synthetic DNA, Collard Greens, Male Gamete, Male Gametophyte, Gene Expression, Differential Display, Transcript Expression Analyses, pollen grain (exact), mitosis, Gamete, Natural Fertility, synthetic, Broccoli, Mitotic M Phase, Determinants, mRNA Differential Display, M Phase, Meiotic M Phases, dJ139D8.5, Gene Expression Monitoring, Phase, Meiotic M, RP1-139D8.5, Transcriptome Profiling, Mitoses, mRNA, Transcriptome Profilings, Plant, Differential Displays, synthetic constructs, Transcriptome Analyses, Preferences, Fertility Determinants, Grains, Differential, SYNTHETIC CONSTRUCT sequences, Plant Male Gametes, Gene Expression Analyses, 9430096I18Rik, Marital, Collard Green, Mitotic M Phases, Surveys, AI429294, artificial, Expression Analysis, Mitotic, Microspore, Fecundability, HSA277276, Fertility Survey, pollen grain, Marital Fertility"],"additional_accession":[]},"is_claimable":false,"name":"Cytological and transcriptome analysis at meiosis and mitosis reveals insights into pollen fertility in synthetic Brassica allohaploid by crosses between B. nigra and B. rapa","description":"Cytological and transcriptome analysis at meiosis and mitosis reveals insights into pollen fertility in synthetic Brassica allohaploid by crosses between B. nigra and B. rapa","dates":{"last_updated":"2023-05-19","first_public":"2023-01-08"},"accession":"PRJNA795975","cross_references":{"GEO":["GSE193368"]}}