{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR179/014/SRR17950714/SRR17950714.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR179/013/SRR17950713/SRR17950713.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR179/015/SRR17950715/SRR17950715.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["sun yat-sen university"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA802278"],"scientific_name":["Homo sapiens"],"long_description":["Purpose: RNA-seq was used to evaluate the effect of CCT2 knockdown in HNSCC cell lines. Methods: CCT2 was knocked down in SAS cell lines through shRNA and then RNA was extracted from control cells and knockdown cells. Results: Using an optimized data analysis workflow, we identified 205 differentially expressed genes (DEG), of which 141 genes were down-regulated and 64 genes up-regulated, respectively (fold change >2 and adjust p-value < 0.05). RT-qPCR was used to confirm the reliability of RNA-seq data. KEGG and GSEA analysis indicated that PI3K/AKT signaling pathway might be involved in the function of CCT2, which had been proved by western blotting. Overall design: CCT2 was knocked down by shRNA in HNSCC cell line-SAS and the RNA was prepared from control and knockdown cell for RNA-seq."],"repository":["ENA"],"name_synonyms":["PRKBA, CCT-beta, Akt/PKB, CG5373, dP60, Vps34, ATVPS34, DAKT1/PKB, Head And Neck, PI3K, BcDNA:LD15217, Dp110, Squamous Cell Carcinoma, AKT1, CT1187, VPS34, 11621, PI-3 kinase, p110-alpha, PHOSPHATIDYLINOSITOL 3-KINASE, DmelCG2699, F8A5.4, BIN3, p110alpha, phosphatidylinositol 3-kinase activity, Dp60, Pi3K92D, CG4141, PI3K_59F, catalyst activity, CG18330, Pi3k, PI3-kinase activity, Head And Neck Squamous Cell Carcinomas, Oral Squamous Cell Carcinomas, PI[[3]]K, PI3Kgamma, PKB|Akt, RacPK, MCAP, p55alpha, PI3K 68D, Pi3K, PI3k, Squamous Cell of Head and Neck, MCMTC, ATP - 1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, Carcinoma, dVps34, PI3K68D, Tcp1-beta, TCP-1-beta, dakt, P110DELTA, DL3610W, PtdIns-3-kinase activity, rea, p50alpha, TCP-1beta, l(3)89Bq, CG2699, DmelCG5373, vacuolar protein sorting 34, dPIK, 1-phosphatidylinositol 3-kinase activity, CCT2, Cct2, PKB/Akt, PKB/AKT, AKT/PKB, C530050K14, dAkt, dAKT, type-1 PI3K, cct2, Oral Squamous Cell Carcinoma, p-Akt, TCP2, DAkt, FCAALL.209, Oropharyngeal Squamous Cell Carcinoma, CG11621, DPKB, pAkt, p85alpha, Laryngeal Squamous Cell Carcinoma, 99D8.1, CCTB, carcinoma of the head and neck, PI3K 68_D, ATCCT2, dAKT/dPKB, PKB/dAKT, DmelCG4141, F8A5_4, APDS, p110D, Dakt, HEL-S-100n, PI3KBETA, dPKB, Cctb, p120-PI3K, droPIK57, RAC-ALPHA, PRO1633, DRAC-PK85, DmelCG4006, PI3K92E, Pi3K_59F, type I phosphatidylinositol kinase activity, phosphatidylinositol 3-kinase, Dpkb, PIK3, dVps34/PI3K59F, Pi3Kp60, p60, PI3K-dp110, dPI3K, PHOSPATIDYLINOSITOL 3-KINASE, anon-92Ed, HNSCC, Dp110/PI3K, PI3K-68D/E, class I, l(3)04226., PI3'K, Pi3Kp110, P110BETA, phosphorylcholine cytidylyltransferase2, PI3K-68D, PI3K21B, AKT, Akt, DmelCG18330, DRAC-PK, MCM, Cpk, dP110, Squamous Cell Carcinoma of the Mouth, 6330412C24Rik, Squamous Cell Carcinoma of the Nasal Cavity, AA414921, akt, p120, class II, type III phosphoinositide 3-kinase activity, Squamous Cell Carcinoma of the Head and Neck, p110, Vps34p, DAkt1, DAKT1, Squamous Cell Carcinoma of the Larynx, PI(3)K, DmelCG7033, cpk, Hypopharyngeal Squamous Cell Carcinoma, PI3K-92E/Dp110, PIK3C1, ATP:1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, PKB, CWS6, vps34, CWS5, PKB-ALPHA, dp110, D-Akt, CG4006, PI3CG, PI-3-K, Dakt1, PI3K-59F, PI3K-Dp110, akt1, dAkt1, DRAC-PK66, class III, Oral Tongue Squamous Cell Carcinoma, DmelCG11621, p110gamma, caPI3K, dAkt/PKB, SD02216p, dakt1, Oral Cavity Squamous Cell Carcinoma, CLOVE, DmVps34, IMD14, PI3K_68D, Squamous Cell Carcinoma of Larynx, PI3K-92D, PKBalpha, Head and Neck Squamous Cell Carcinoma, Dmp110, dAKT1, RAC, Rac"],"description_synonyms":["PRKBA, CCT-beta, Akt/PKB, CG5373, dP60, Vps34, ATVPS34, DAKT1/PKB, Head And Neck, PI3K, BcDNA:LD15217, Dp110, Squamous Cell Carcinoma, AKT1, CT1187, VPS34, 11621, PI-3 kinase, p110-alpha, PHOSPHATIDYLINOSITOL 3-KINASE, DmelCG2699, F8A5.4, BIN3, p110alpha, phosphatidylinositol 3-kinase activity, Dp60, Pi3K92D, CG4141, PI3K_59F, catalyst activity, CG18330, Pi3k, PI3-kinase activity, Head And Neck Squamous Cell Carcinomas, Oral Squamous Cell Carcinomas, PI[[3]]K, PI3Kgamma, PKB|Akt, RacPK, MCAP, p55alpha, PI3K 68D, Pi3K, PI3k, Squamous Cell of Head and Neck, MCMTC, ATP - 1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, Carcinoma, dVps34, PI3K68D, Tcp1-beta, TCP-1-beta, dakt, P110DELTA, DL3610W, PtdIns-3-kinase activity, rea, p50alpha, TCP-1beta, l(3)89Bq, CG2699, DmelCG5373, vacuolar protein sorting 34, dPIK, 1-phosphatidylinositol 3-kinase activity, CCT2, Cct2, PKB/Akt, PKB/AKT, AKT/PKB, C530050K14, dAkt, dAKT, type-1 PI3K, cct2, Oral Squamous Cell Carcinoma, p-Akt, TCP2, DAkt, FCAALL.209, Oropharyngeal Squamous Cell Carcinoma, CG11621, DPKB, pAkt, p85alpha, Laryngeal Squamous Cell Carcinoma, 99D8.1, CCTB, carcinoma of the head and neck, PI3K 68_D, ATCCT2, dAKT/dPKB, PKB/dAKT, DmelCG4141, F8A5_4, APDS, p110D, Dakt, HEL-S-100n, PI3KBETA, dPKB, Cctb, p120-PI3K, droPIK57, RAC-ALPHA, PRO1633, DRAC-PK85, DmelCG4006, PI3K92E, Pi3K_59F, type I phosphatidylinositol kinase activity, phosphatidylinositol 3-kinase, Dpkb, PIK3, dVps34/PI3K59F, Pi3Kp60, p60, PI3K-dp110, dPI3K, PHOSPATIDYLINOSITOL 3-KINASE, anon-92Ed, HNSCC, Dp110/PI3K, PI3K-68D/E, class I, l(3)04226., PI3'K, Pi3Kp110, P110BETA, phosphorylcholine cytidylyltransferase2, PI3K-68D, PI3K21B, AKT, Akt, DmelCG18330, DRAC-PK, MCM, Cpk, dP110, Squamous Cell Carcinoma of the Mouth, 6330412C24Rik, Squamous Cell Carcinoma of the Nasal Cavity, AA414921, akt, p120, class II, type III phosphoinositide 3-kinase activity, Squamous Cell Carcinoma of the Head and Neck, p110, Vps34p, DAkt1, DAKT1, Squamous Cell Carcinoma of the Larynx, PI(3)K, DmelCG7033, cpk, Hypopharyngeal Squamous Cell Carcinoma, PI3K-92E/Dp110, PIK3C1, ATP:1-phosphatidyl-1D-myo-inositol 3-phosphotransferase activity, PKB, CWS6, vps34, CWS5, PKB-ALPHA, dp110, D-Akt, CG4006, PI3CG, PI-3-K, Dakt1, PI3K-59F, PI3K-Dp110, akt1, dAkt1, DRAC-PK66, class III, Oral Tongue Squamous Cell Carcinoma, DmelCG11621, p110gamma, caPI3K, dAkt/PKB, SD02216p, dakt1, Oral Cavity Squamous Cell Carcinoma, CLOVE, DmVps34, IMD14, PI3K_68D, Squamous Cell Carcinoma of Larynx, PI3K-92D, PKBalpha, Head and Neck Squamous Cell Carcinoma, Dmp110, dAKT1, RAC, Rac"],"additional_accession":[]},"is_claimable":false,"name":"CCT2 promotes the proliferation and migration of head and neck squamous cell carcinoma via activating PI3K/AKT pathway","description":"CCT2 promotes the proliferation and migration of head and neck squamous cell carcinoma via activating PI3K/AKT pathway","dates":{"last_updated":"2025-09-24","first_public":"2022-02-05"},"accession":"PRJNA802278","cross_references":{"GEO":["GSE195850"],"taxon":["9606"]}}