ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/051/SRR18664451/SRR18664451_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/054/SRR18664454/SRR18664454_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/055/SRR18664455/SRR18664455_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/056/SRR18664456/SRR18664456_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/049/SRR18664449/SRR18664449_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/050/SRR18664450/SRR18664450_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/051/SRR18664451/SRR18664451_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/052/SRR18664452/SRR18664452_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/054/SRR18664454/SRR18664454_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/053/SRR18664453/SRR18664453_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/049/SRR18664449/SRR18664449_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/052/SRR18664452/SRR18664452_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/056/SRR18664456/SRR18664456_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/053/SRR18664453/SRR18664453_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/050/SRR18664450/SRR18664450_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR186/055/SRR18664455/SRR18664455_1.fastq.gzprimaryOK200GenomicsMilla Lab, Pediatrics - Pulmonary Medicine, Stanford Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA824119Homo sapiensRespiratory epithelium interact with our microbiome as well as environmental bacteria, and are critical in maintaining homeostasis in face of disruption such as injury or infection. Here we investigate the impact of a filamentous bacteriophage on responses of these cells to bacterial stimulus. Overall design: Single-cell suspensions of basal cell culture were prepared by lifting adherent cells using trypsin digestion. Dead cells were removed using the MACS Dead Cell Removal kit (Miltenyi Biotech). Cells were then washed and counted as per manufacturer’s instructions and libraries were prepared using the 10X NEXT GEM 3’ Gene Expression Library scRNA Seq v3.1 reagents and workflow (10x Genomics). Library concentration and quality was confirmed by BioAnalyzer. Libraries were sequenced on the HiSeq PE150 platform.xref:PubMed:38275975ENACystic fibrosis NOS, pseudomonas aeruginosa, h-BCS, dTAF[[II]]230, TAF[[II]]250, d230, cystic fibrosis with pulmonary manifestations (disorder)., BJS, susceptibility to chronic infection by, FLNMS, Meconium obstruction of intestine in mucoviscidosis, TAF200, Mucoviscidosis, Hs.6719, Gene, l(3)84Ab, Cystic fibrosis NOS (disorder), dTAFII250, Pancreas Fibrocystic Diseases, BG:DS00004.13, TAFII-250, TAF250/230, EfW1, Client, Cell, CF - Cystic fibrosis, dTAF230, BCS1, dmTAF[[II]]230, cystic fibrosis with pulmonary manifestations, TAFII250, dmTAF1, Taf230, p230, CYSTIC FIBROS W/O ILEUS, TAF[[II]]250/230, TFIID, cystic fibrosis with other manifestations (disorder), BCS, cystic fibrosis lung disease, Pancreatic, Fibrosis, modifier of, Meconium ileus in cystic fibrosis (disorder), Pulmonary, TAF250, in cystic fibrosis, Taf[[II]]250, BC, PTD, Cystic fibrosis (disorder), Taf200, Pancreatic Cystic Fibrosis, dTAF[[II]]250, TAF[[II]]230, MC3DN1, Gene Expressions, cystic fibrosis with meconium ileus, TFIID TAF250, cel, Cystic Fibrosis of Pancreas, cell, cystic fibrosis with other manifestations, Cystic fibrosis without mention of meconium ileus, BCs, Taf1p, TAF[II]250, cystic fibrosis with combined manifestations, cystic fibrosis, CG17603, cystic fibrosis with meconium ileus (disorder), Expressions, TAF[[II]], cystic fibrosis with gastrointestinal manifestations, Fibrocystic Disease of Pancreas, Fibrocystic disease, dTAF250, Cystic, CF, BPs, Cystic Fibrosis, DmelCG17603, Taf250, Patient, SR3-5, mucoviscidosis, Clients, GRACILE, Pancreas Fibrocystic Disease, Pulmonary Cystic Fibrosis, Expression, TAF, TAF230, TAF1Cystic fibrosis NOS, pseudomonas aeruginosa, h-BCS, dTAF[[II]]230, TAF[[II]]250, d230, cystic fibrosis with pulmonary manifestations (disorder)., BJS, susceptibility to chronic infection by, FLNMS, Meconium obstruction of intestine in mucoviscidosis, TAF200, Mucoviscidosis, Hs.6719, Gene, l(3)84Ab, Cystic fibrosis NOS (disorder), dTAFII250, Pancreas Fibrocystic Diseases, BG:DS00004.13, TAFII-250, TAF250/230, EfW1, Client, Cell, CF - Cystic fibrosis, dTAF230, BCS1, dmTAF[[II]]230, cystic fibrosis with pulmonary manifestations, TAFII250, dmTAF1, Taf230, p230, CYSTIC FIBROS W/O ILEUS, TAF[[II]]250/230, TFIID, cystic fibrosis with other manifestations (disorder), BCS, cystic fibrosis lung disease, Pancreatic, Fibrosis, modifier of, Meconium ileus in cystic fibrosis (disorder), Pulmonary, TAF250, in cystic fibrosis, Taf[[II]]250, BC, PTD, Cystic fibrosis (disorder), Taf200, Pancreatic Cystic Fibrosis, dTAF[[II]]250, TAF[[II]]230, MC3DN1, Gene Expressions, cystic fibrosis with meconium ileus, TFIID TAF250, cel, Cystic Fibrosis of Pancreas, cell, cystic fibrosis with other manifestations, Cystic fibrosis without mention of meconium ileus, BCs, Taf1p, TAF[II]250, cystic fibrosis with combined manifestations, cystic fibrosis, CG17603, cystic fibrosis with meconium ileus (disorder), Expressions, TAF[[II]], cystic fibrosis with gastrointestinal manifestations, Fibrocystic Disease of Pancreas, Fibrocystic disease, dTAF250, Cystic, CF, BPs, Cystic Fibrosis, DmelCG17603, Taf250, Patient, SR3-5, mucoviscidosis, Clients, GRACILE, Pancreas Fibrocystic Disease, Pulmonary Cystic Fibrosis, Expression, TAF, TAF230, TAF1falseGene expression profile at single cell level of Basal Cells (BCs) from nasal scrapings of patients with and without cystic fibrosisGene expression profile at single cell level of Basal Cells (BCs) from nasal scrapings of patients with and without cystic fibrosis2025-09-242022-07-06PRJNA824119GSE200342960638275975