{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR188/074/SRR18885474/SRR18885474.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR188/073/SRR18885473/SRR18885473.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Sun Yan-Sen University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA830705"],"scientific_name":["Homo sapiens"],"long_description":["Purpose: To verify the relationship between AUF1 and tumor cells proliferation, we detected the function of AUF1 in Hela cells using RIP-seq analysis. Methods: Genomic alignment (version from UCSC genome browser) was using Tophat (version:2.0.13) to get uniquely mapping reads. p value <0.05 considered as significantly modulated, were retained for further analysis. This choice is motivated by the decision to maximize the sensitivity of this analysis, in order to perform a massive screening and identify candidate genes to be validated with a wider sample population with real-time PCR analysis Results: The quantity of gene expression was calculated by FPKM (Fragments Per Kilobase of transcript per Million fragments mapped).50% of the binding region of AUF1 was in the exon region, and others were mainly in the 3’-UTR regions (29.6%). AUF1’s targeted genes were related to metabolic and single-organism processes. AUF1’s targeted genes were mainly concentrated in the protein synthesis process of the endoplasmic reticulum and cancer pathway. Conclusions: AUF1 might be involved in the regulation of translation and participated in the regulation of tumorigenesis and development through its target genes. Overall design: Fragment of RNA binding to AUF1 antibody in Hela cells"],"repository":["ENA"],"description_synonyms":["biochemical pathways, Polyadenylated Messenger RNA, cellular breakdown, RNA, Poly(A)+ RNA, RIP-seq, Non Polyadenylated mRNA, protein_coding_transcript, biodegradation, degradation, mRNA, Poly(A) Tail, breakdown of chemical, Polyadenylated, catabolism, ligand, Polyadenylated Messenger, Non-Polyadenylated, RIP-seq assay., Messenger RNA, messenger RNA, cellular catabolism, Non-Polyadenylated mRNA, breakdown of substance, template RNA, Messenger, Poly(A)+ mRNA, INSDC_feature:mRNA, cellular degradation, biotransformation, secretion, Polyadenylated RNA, Polyadenylated mRNA, Non Polyadenylated, Poly(A) RNA, breakdown of molecule"],"name_synonyms":["biochemical pathways, Polyadenylated Messenger RNA, cellular breakdown, RNA, Poly(A)+ RNA, RIP-seq, Non Polyadenylated mRNA, protein_coding_transcript, biodegradation, degradation, mRNA, Poly(A) Tail, breakdown of chemical, Polyadenylated, catabolism, ligand, Polyadenylated Messenger, Non-Polyadenylated, RIP-seq assay., Messenger RNA, messenger RNA, cellular catabolism, Non-Polyadenylated mRNA, breakdown of substance, template RNA, Messenger, Poly(A)+ mRNA, INSDC_feature:mRNA, cellular degradation, biotransformation, secretion, Polyadenylated RNA, Polyadenylated mRNA, Non Polyadenylated, Poly(A) RNA, breakdown of molecule"],"additional_accession":[]},"is_claimable":false,"name":"A novel strategy for regulating mRNA’s degradation via interfering the AUF1’s binding to mRNA [RIP-seq]","description":"A novel strategy for regulating mRNA’s degradation via interfering the AUF1’s binding to mRNA [RIP-seq]","dates":{"last_updated":"2025-09-24","first_public":"2022-06-16"},"accession":"PRJNA830705","cross_references":{"GEO":["GSE201279"],"taxon":["9606"]}}