{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["WASHINGTON UNIVERSITY"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA835195"],"scientific_name":["Homo sapiens"],"long_description":["This study involved prospective collection of skin biopsies and peripheral blood mononuclear cell (PBMC) samples from controls with no self-reported history of autoimmune or inflammatory disease and cases with Rheumatologist-confirmed systemic sclerosis: limited cutaneous systemic sclerosis (lcSSc), diffuse cutaneous systemic sclerosis (dcSSc), very early diagnosis of systemic sclerosis (VEDOSS), and systemic sclerosis sine scleroderma (SSS). Some SSc cases were sampled at a 6 month routine follow-up visit. PBMCs were collected and lysed immediately. Skin punches were stabilized in RNAlater until extraction. All RNA-Seq libraries were created using Takara/Clontech ribosomal depletion kits, and sequenced on either a HiSeq2500 or HiSeq3000."],"repository":["ENA"],"description_synonyms":["skin benign neoplasm, dermis plus epidermis plus hypodermis, the integument, region of skin, Oct-11a, skin, Systemic Sclerosis, total RNA extract, pelt, Systemic, Otf-11, Skn-li, Sclerosis, vertebrate integument, dermoid system, entire skin, skin zone, integumental organ, SKIN, Scleroderma, mKIAA0493, Epoc-1, epidermis, skin organ, portion of skin, skin plus hypodermis, vertebrate epidermis, Oct11, skin region, Client., integument, Patient, Clients, BC033609, Fam91a1, Otf11, Skin-1a, entire integument, Systemic sclerosis, tegument, AV220772, Skin, dermal system, skin and subcutaneous tissue, Systemic Scleroderma, integumentum commune, Skn-1a"],"name_synonyms":["skin benign neoplasm, dermis plus epidermis plus hypodermis, the integument, region of skin, Oct-11a, skin, Systemic Sclerosis, total RNA extract, pelt, Systemic, Otf-11, Skn-li, Sclerosis, vertebrate integument, dermoid system, entire skin, skin zone, integumental organ, SKIN, Scleroderma, mKIAA0493, Epoc-1, epidermis, skin organ, portion of skin, skin plus hypodermis, vertebrate epidermis, Oct11, skin region, Client., integument, Patient, Clients, BC033609, Fam91a1, Otf11, Skin-1a, entire integument, Systemic sclerosis, tegument, AV220772, Skin, dermal system, skin and subcutaneous tissue, Systemic Scleroderma, integumentum commune, Skn-1a"],"additional_accession":[]},"is_claimable":false,"name":"Ribosomal Depleted Total RNA-Seq of PBMCs and Skin from Controls and Systemic Sclerosis Patients","description":"Ribosomal Depleted Total RNA-Seq of PBMCs and Skin from Controls and Systemic Sclerosis Patients","dates":{"last_updated":"2025-09-24","first_public":"2022-05-25"},"accession":"PRJNA835195","cross_references":{"taxon":["9606"]}}