ENAapplication/xmlftp://primaryOK200GenomicsWASHINGTON UNIVERSITYhttps://www.ebi.ac.uk/ena/browser/view/PRJNA835195Homo sapiensThis study involved prospective collection of skin biopsies and peripheral blood mononuclear cell (PBMC) samples from controls with no self-reported history of autoimmune or inflammatory disease and cases with Rheumatologist-confirmed systemic sclerosis: limited cutaneous systemic sclerosis (lcSSc), diffuse cutaneous systemic sclerosis (dcSSc), very early diagnosis of systemic sclerosis (VEDOSS), and systemic sclerosis sine scleroderma (SSS). Some SSc cases were sampled at a 6 month routine follow-up visit. PBMCs were collected and lysed immediately. Skin punches were stabilized in RNAlater until extraction. All RNA-Seq libraries were created using Takara/Clontech ribosomal depletion kits, and sequenced on either a HiSeq2500 or HiSeq3000.ENAskin benign neoplasm, dermis plus epidermis plus hypodermis, the integument, region of skin, Oct-11a, skin, Systemic Sclerosis, total RNA extract, pelt, Systemic, Otf-11, Skn-li, Sclerosis, vertebrate integument, dermoid system, entire skin, skin zone, integumental organ, SKIN, Scleroderma, mKIAA0493, Epoc-1, epidermis, skin organ, portion of skin, skin plus hypodermis, vertebrate epidermis, Oct11, skin region, Client., integument, Patient, Clients, BC033609, Fam91a1, Otf11, Skin-1a, entire integument, Systemic sclerosis, tegument, AV220772, Skin, dermal system, skin and subcutaneous tissue, Systemic Scleroderma, integumentum commune, Skn-1askin benign neoplasm, dermis plus epidermis plus hypodermis, the integument, region of skin, Oct-11a, skin, Systemic Sclerosis, total RNA extract, pelt, Systemic, Otf-11, Skn-li, Sclerosis, vertebrate integument, dermoid system, entire skin, skin zone, integumental organ, SKIN, Scleroderma, mKIAA0493, Epoc-1, epidermis, skin organ, portion of skin, skin plus hypodermis, vertebrate epidermis, Oct11, skin region, Client., integument, Patient, Clients, BC033609, Fam91a1, Otf11, Skin-1a, entire integument, Systemic sclerosis, tegument, AV220772, Skin, dermal system, skin and subcutaneous tissue, Systemic Scleroderma, integumentum commune, Skn-1afalseRibosomal Depleted Total RNA-Seq of PBMCs and Skin from Controls and Systemic Sclerosis PatientsRibosomal Depleted Total RNA-Seq of PBMCs and Skin from Controls and Systemic Sclerosis Patients2025-09-242022-05-25PRJNA8351959606