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k/vol1/fastq/SRR191/047/SRR19185147/SRR19185147_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR191/041/SRR19185141/SRR19185141_2.fastq.gzprimaryOK200GenomicsHartig Laboratory, Medicine, Baylor College of Medicinehttps://www.ebi.ac.uk/ena/browser/view/PRJNA837777Mus musculusxref:PubMed:36243005Low-grade, sustained inflammation in white adipose tissue (WAT) characterizes obesity and frequently coincides with insulin resistance and type 2 diabetes (T2D). However, pharmacological targeting of WAT inflammation lacks durable therapeutic effects. Through a computational screen, we identified the FDA-approved rheumatoid arthritis drug auranofin is a putative small molecule for obesity treatment. We discovered that allometrically scaled safe auranofin doses homed to WAT and improved insulin sensitivity in obese wild-type mice. To assess the transcriptional changes underlying the beneficial effects of auranofin, we performed RNA-seq analysis on adipose and liver tissue after 4 weeks of treatment with vehicle or auranofin. Overall design: Comparative gene expression profiling analysis of RNA-seq data for liver, epididymal and inguinal white adipose tissue, and intrascapular brown adipose tissue from C57Bl/6 mice. Mice were ffed 60% HFD diet for 12 weeks before receiving vehicle or auranofin (1 mg/kg BW) 3 times per week for 4 weeks.ENASK&F-39162, SK&F D 39162, BODYFAT, Ob Gene Product, Occidental, Ridaura, AW413978, adipose system, (1-thio-beta-D-glucopyranose 2, Tissue, white, Gm695, fat, Ob., Gene Product, SK&F 39162, Ridauran, Gold, Obese, Obese Gene Product, Body Fat, Crisinor, Caucasian, Abc8, fatty depot, Whites, adipose, European, adp, bodyfat, 3, White, 4, 6-tetraacetato-S)(triethylphosphine)-, fatty tissue, Ob Protein, Caucasoid, fat tissue, Obese Protein, SK&F39162, CaucasiansSK&F-39162, SK&F D 39162, BODYFAT, Ob Gene Product, Occidental, Ridaura, AW413978, adipose system, (1-thio-beta-D-glucopyranose 2, Tissue, white, Gm695, fat, Ob., Gene Product, SK&F 39162, Ridauran, Gold, Obese, Obese Gene Product, Body Fat, Crisinor, Caucasian, Abc8, fatty depot, Whites, adipose, European, adp, bodyfat, 3, White, 4, 6-tetraacetato-S)(triethylphosphine)-, fatty tissue, Ob Protein, Caucasoid, fat tissue, Obese Protein, SK&F39162, CaucasiansfalseAccumulation of auranofin in white adipose tissues lowers leptin levels and exerts anti-diabetic effectsAccumulation of auranofin in white adipose tissues lowers leptin levels and exerts anti-diabetic effects2025-09-242022-10-08PRJNA837777GSE2029351009036243005