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Weatherall Institute of Molecular Medicinehttps://www.ebi.ac.uk/ena/browser/view/PRJNA850076Homo sapiensxref:PubMed:37267382Skin and blood single cell RNA sequencing datasets of IL-22- and IFNγ-secreting CD1a-reactive GAS-responsive T cells were generated from four healthy skins, five healthy PBMCs and three psoriatic PBMCs. Using cellular indexing of transcriptomes and epitopes by sequencing (CITE-seq), we measured T cell specific functional states at both protein and transcriptome levels. Overall design: We deployed CD1a-expressing K562 cells as antigen presenting cells. K562-CD1a were infected with GAS, and were recognized by ex vivo cutaneous and blood T cells in a CD1a-dependent manner leading to production of IL-22 or IFNγ, detected using Cytokine Secretion assays. FACS-isolated CD3+ T cells with or without prior K562 co-culture were included to establish the dataset. CITE-seq antibodies against the fluorochromes PE or APC on the detection antibodies were included to further characterise cytokine-producing T cells.ENAfalseSingle cell analysis of CD1a-restricted Group A streptococcus (GAS)-reactive peripheral and cutaneous T cellsSingle cell analysis of CD1a-restricted Group A streptococcus (GAS)-reactive peripheral and cutaneous T cells2025-09-242023-07-13PRJNA850076GSE206326960637267382