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for Neurodegenerative Diseases, University of California San Francisco"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA853730"],"scientific_name":["Homo sapiens"],"long_description":["We performed a pooled CRISPRi screen (CROP-seq) and genome editing to validate 19 genetic variants prioritized from massively parallel reporter assays to screen 5,706 polymorphisms from genome-wide association studies for both Alzheimer’s disease (AD) and Progressive Supranuclear Palsy (PSP) across 11 distinct loci. This allowed us to pinpoint regulatory targets in a cell-type specific manner. Overall design: sgRNAs were created targeting putative cis-regulatory regions and were transfected into iPSC-derived iNeurons and iMicroglia containing CRISPRi machinery. These were then collected for 10X sequencing using the CROPseq method."],"repository":["ENA"],"name_synonyms":["PSP-94, PSP57, PSP1, ahl4, P14.5, Protein unc-18 homolog C, HPC13, PSP94, Steele-Richardson-Olszewski Syndrome, Hrp12, Nerve, Short palate, Platelet Sec1 protein, 2610511A05Rik, PSPase, 1-benzoxathiole 1, Cell, PRPS, BB234005, Microglial, 3.1.3.3, PSPS1, O-phosphoserine phosphohydrolase, iPSC, UNC-18C, Psp, PSP, Cis, CIS, G18, Steele-Richardson-Olszewski syndrome, 1-benzoxathiol-3-ylidene)bisphenol S, 3, 4, iPS cell, Microglias, alpha-hydroxy-alpha, MSPB, alpha-bis(p-hydroxyphenyl)-o-toluenesulfonic acid gamma-sultone, 1-dioxide, IGBF, PSNP1, Phenolsulfonphthalein, MF.microglia.CNS, CIS-1, microglial cell, Nerve Cells, SPLUNC2, MUNC18C, Hortega cell, brain-resident macrophage., PTP, 4'-(3H-2, pneumothorax, HRSP12, Microglial Cell, Neonatal submandibular gland protein, 9030605P22Rik, Unc-18C, Bpifa2e, PN44, Parotid secretory protein, microgliocyte, microglia, PSPS, bA49G10.1, Unc18-3, MUNC18-3, PSPHD, lung and nasal epithelium carcinoma-associated protein 2, SOCS, MSP, ICRF, UK114, Cells, L-3-phosphoserine phosphatase, AI480570, Protein G18, 3-bis(p-hydroxyphenyl)-3H-2, Neuron, Progressive Supranuclear Palsy, brain macrophage, hortega cells, REG, Protein unc-18 homolog 3, S-dioxide, primary spontaneous pneumothorax, associated, P19, Suppressor of cytokine signaling, Nerve Cell, primary spontaneous, C20orf70, spontaneous pneumothorax, BACTS2, Microglial Cells"],"description_synonyms":["PSP-94, PSP57, PSP1, ahl4, P14.5, Protein unc-18 homolog C, HPC13, PSP94, Steele-Richardson-Olszewski Syndrome, Hrp12, Nerve, Short palate, Platelet Sec1 protein, 2610511A05Rik, PSPase, 1-benzoxathiole 1, Cell, PRPS, BB234005, Microglial, 3.1.3.3, PSPS1, O-phosphoserine phosphohydrolase, iPSC, UNC-18C, Psp, PSP, Cis, CIS, G18, Steele-Richardson-Olszewski syndrome, 1-benzoxathiol-3-ylidene)bisphenol S, 3, 4, iPS cell, Microglias, alpha-hydroxy-alpha, MSPB, alpha-bis(p-hydroxyphenyl)-o-toluenesulfonic acid gamma-sultone, 1-dioxide, IGBF, PSNP1, Phenolsulfonphthalein, MF.microglia.CNS, CIS-1, microglial cell, Nerve Cells, SPLUNC2, MUNC18C, Hortega cell, brain-resident macrophage., PTP, 4'-(3H-2, pneumothorax, HRSP12, Microglial Cell, Neonatal submandibular gland protein, 9030605P22Rik, Unc-18C, Bpifa2e, PN44, Parotid secretory protein, microgliocyte, microglia, PSPS, bA49G10.1, Unc18-3, MUNC18-3, PSPHD, lung and nasal epithelium carcinoma-associated protein 2, SOCS, MSP, ICRF, UK114, Cells, L-3-phosphoserine phosphatase, AI480570, Protein G18, 3-bis(p-hydroxyphenyl)-3H-2, Neuron, Progressive Supranuclear Palsy, brain macrophage, hortega cells, REG, Protein unc-18 homolog 3, S-dioxide, primary spontaneous pneumothorax, associated, P19, Suppressor of cytokine signaling, Nerve Cell, primary spontaneous, C20orf70, spontaneous pneumothorax, BACTS2, Microglial Cells"],"additional_accession":[]},"is_claimable":false,"name":"CROPSeq of Putative AD- and PSP-associated cis-Regulatory Regions in iPSC-derived Neurons and Microglia","description":"CROPSeq of Putative AD- and PSP-associated cis-Regulatory Regions in iPSC-derived Neurons and Microglia","dates":{"last_updated":"2025-09-24","first_public":"2022-07-06"},"accession":"PRJNA853730","cross_references":{"GEO":["GSE207099"],"taxon":["9606"]}}