ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR212/094/SRR21201594/SRR21201594_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR212/095/SRR21201595/SRR21201595_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR212/096/SRR21201596/SRR21201596_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR212/093/SRR21201593/SRR21201593_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR212/093/SRR21201593/SRR21201593_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR212/094/SRR21201594/SRR21201594_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR212/095/SRR21201595/SRR21201595_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR212/096/SRR21201596/SRR21201596_1.fastq.gzprimary200OKGenomicsUniversity of Chicagohttps://www.ebi.ac.uk/ena/browser/view/PRJNA873688Homo sapiensxref:PubMed:37478845The FTO gene locus has been linked to cancer and obesity through encoded N6-methyladenosine (m6A) demethylase FTO or inherited genomic variants (e.g. intronic single-nucleotide polymorphisms). Here we demonstrate that FTO-IT1, a long noncoding RNA (lncRNA) transcribed from a FTO gene intron, is upregulated during prostate cancer (PCa) progression and positively correlated with poor survival of patients with tumors only expressing wild-type p53. We show that RBM15, a mRNA/substrate binding subunit of the m6A methyltransferase complex binds and increases mRNA m6A methylation and stability of p53 transcriptional target genes however, FTO-IT1 overexpression abolishes these effects by blocking RBM15 binding of p53 target gene mRNAs. Therapeutic targeting of FTO-IT1 restores mRNA m6A level and p53 signaling and inhibits PCa tumor growth in mice. Our study identifies FTO-IT1 lncRNA as a bono fide inhibitor of m6A methylation and p53 tumor suppression and nominates FTO-IT1 as a potential biomarker and therapeutic target of cancer. Overall design: [Dataset 1] 4 samples. Duplicates for CLIP-Seq samples with an antibody against RBM15 in 22Rv1 (CRL-2505) human prostate carcinoma cell lineENAbeta[[3]]-Tub, Non-Translated RNA, DmelCG17117, dp53, p50, LINC RNA, ALKBH9, Neoplasms, p53, Long Non-Translated, Benign Neoplasm, GPM6, Gpm6, Long ncRNAs, 143391_i_at, beta3 TU, Tumor, Long Intergenic Non Protein Coding RNA, LFS1, betaTub3, Malignant, Tp53, l(3)05745, Noncoding RNA, Long Non Translated, DMP53, Messenger, bbl, Long Non-Translated RNA, AA959943, Alleviating interaction, Dmp53, LincRNAs, anon-EST:Liang-2.13, Non Polyadenylated, Long Non-Protein-Coding, Long Non-Coding, lncRNA, 1323/07, clone 2.13, BCC7, Malignancy, Non-Protein-Coding RNA, D.m.BETA-60D, Polyadenylated Messenger, beta-Tub6D, dmp53, inhibiteur, messenger RNA, T, DmP53, 1422/04, INSDC_feature:ncRNA, Acts, Neoplasias, N(6)mAdo, B3t, template RNA, DmelCG3401, Trp53, inhibidor, Long Non-Protein-Coding RNA, N(6)-methyladenosine, Dp53, betaTub60C, beta3-tubulin, Malignancies, beta[[3]]-tubulin, TRP53, CG17117, Long Noncoding RNA, Methylations, Long Untranslated, p50/tubulin, Long Untranslated RNA, Cancer, Tumors, Acta-2, inhibitors, beta-Tub60D, CG10873, RNA, Long Non-Coding RNA, Dmbeta3, Dm-HTH, Malignant Neoplasm, Polyadenylated, ncRNA, ncRNAs, AW743446, BETA 60D, prac, N6-methyladenosine (m6A), IME4, beta3t, beta3-Tub, Actsk-1, Messenger RNA, inhibitor, Long ncRNA, Xp53, m(6)A, 3t, Benign, Non-Coding RNA, Poly(A)+ mRNA, p53/tubulin, MT-A70, Neoplasm, INSDC_feature:mRNA, Long Non Protein Coding RNA, bfy, Polyadenylated RNA, methylation, beta[[3]] tubulin, Long, Untranslated RNA, N6-methyladenosine, RGD1305121, Tub60D, HTH, Hth, suppressive genetic interaction (sensu inequality)., Long Non Coding RNA, antagonists, Polyadenylated Messenger RNA, Spo8, CG33336, beta-tub, Poly(A)+ RNA, Non Polyadenylated mRNA, protein_coding_transcript, mRNA, Poly(A) Tail, DmelCG33336, D-p53, Non-Polyadenylated, beta60C, CG3401, beta3Tub, beta3TUB, Benign Neoplasms, Dm-P53, l(3)86Ca, beta3, Cancers, M6A, Non-Polyadenylated mRNA, dtl, 6-methyladenosine, Malignant Neoplasms, Tub, DTB3, 2310024F18Rik, Meis1, hth1, Long Intergenic Non-Protein Coding RNA, hth2, antagonists and inhibitors, betatub60D, P53, LincRNA, p44, bhy, mKIAA1752, Polyadenylated mRNA, M6a, m6A, other neoplasm, Poly(A) RNA, CG31325, Neoplasia, betaTubbeta[[3]]-Tub, Non-Translated RNA, DmelCG17117, dp53, p50, LINC RNA, ALKBH9, Neoplasms, p53, Long Non-Translated, Benign Neoplasm, GPM6, Gpm6, Long ncRNAs, 143391_i_at, beta3 TU, Tumor, Long Intergenic Non Protein Coding RNA, LFS1, betaTub3, Malignant, Tp53, l(3)05745, Noncoding RNA, Long Non Translated, DMP53, Messenger, bbl, Long Non-Translated RNA, AA959943, Alleviating interaction, Dmp53, LincRNAs, anon-EST:Liang-2.13, Non Polyadenylated, Long Non-Protein-Coding, Long Non-Coding, lncRNA, 1323/07, clone 2.13, BCC7, Malignancy, Non-Protein-Coding RNA, D.m.BETA-60D, Polyadenylated Messenger, beta-Tub6D, dmp53, inhibiteur, messenger RNA, T, DmP53, 1422/04, INSDC_feature:ncRNA, Acts, Neoplasias, N(6)mAdo, B3t, template RNA, DmelCG3401, Trp53, inhibidor, Long Non-Protein-Coding RNA, N(6)-methyladenosine, Dp53, betaTub60C, beta3-tubulin, Malignancies, beta[[3]]-tubulin, TRP53, CG17117, Long Noncoding RNA, Methylations, Long Untranslated, p50/tubulin, Long Untranslated RNA, Cancer, Tumors, Acta-2, inhibitors, beta-Tub60D, CG10873, RNA, Long Non-Coding RNA, Dmbeta3, Dm-HTH, Malignant Neoplasm, Polyadenylated, ncRNA, ncRNAs, AW743446, BETA 60D, prac, N6-methyladenosine (m6A), IME4, beta3t, beta3-Tub, Actsk-1, Messenger RNA, inhibitor, Long ncRNA, Xp53, m(6)A, 3t, Benign, Non-Coding RNA, Poly(A)+ mRNA, p53/tubulin, MT-A70, Neoplasm, INSDC_feature:mRNA, Long Non Protein Coding RNA, bfy, Polyadenylated RNA, methylation, beta[[3]] tubulin, Long, Untranslated RNA, N6-methyladenosine, RGD1305121, Tub60D, HTH, Hth, suppressive genetic interaction (sensu inequality)., Long Non Coding RNA, antagonists, Polyadenylated Messenger RNA, Spo8, CG33336, beta-tub, Poly(A)+ RNA, Non Polyadenylated mRNA, protein_coding_transcript, mRNA, Poly(A) Tail, DmelCG33336, D-p53, Non-Polyadenylated, beta60C, CG3401, beta3Tub, beta3TUB, Benign Neoplasms, Dm-P53, l(3)86Ca, beta3, Cancers, M6A, Non-Polyadenylated mRNA, dtl, 6-methyladenosine, Malignant Neoplasms, Tub, DTB3, 2310024F18Rik, Meis1, hth1, Long Intergenic Non-Protein Coding RNA, hth2, antagonists and inhibitors, betatub60D, P53, LincRNA, p44, bhy, mKIAA1752, Polyadenylated mRNA, M6a, m6A, other neoplasm, Poly(A) RNA, CG31325, Neoplasia, betaTubfalseA lncRNA from the FTO locus acts as an inhibitor of mRNA m6A methylation and p53 tumor suppressionA lncRNA from the FTO locus acts as an inhibitor of mRNA m6A methylation and p53 tumor suppression2025-09-242023-07-10PRJNA873688GSE212043960637478845