ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/051/SRR21588451/SRR21588451.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/055/SRR21588455/SRR21588455.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/048/SRR21588448/SRR21588448.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/050/SRR21588450/SRR21588450.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/056/SRR21588456/SRR21588456.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/046/SRR21588446/SRR21588446.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/045/SRR21588445/SRR21588445.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/053/SRR21588453/SRR21588453.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/047/SRR21588447/SRR21588447.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/054/SRR21588454/SRR21588454.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/052/SRR21588452/SRR21588452.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR215/049/SRR21588449/SRR21588449.fastq.gzprimaryOK200GenomicsGuizhou Medical Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA879946In the current work, we aimed to investigate the antibiofilm of AMP-17 in C.albicans and explored the underlying mechanism. Antifungal susceptibility assay showed AMP-17 exerted a strong in-hibitory efficacy on both biofilm formation and preformed biofilms in C.albicans. RNA-seq was applied to elucidate the underlying molecular mechanism. RNA-seq results showed that MAPK pathway, biosynthesis of antibiotics and essential components of cell were mainly enriched in the biofilm forming stage, while citrate cycle (TCA cycle), phenyla-mine metabolism, propanoate metabolism enriched after biofilm mature. Moreover, the co-expressed DEGs in the two pairwise highlighted the terms of transmembrane transporter activity, regulation of filamentation and biofilm formation acted an important role in the antibiofilm effect of AMP-17. Additionally, qRT-PCR confirmed the level of the genes involved cell adhesion, fila-mentous growth, MAPK, biofilm matrix and cells dispersal correspondingly changed after AMP-17 treatment. Our fundings reveal the underlying antibiofilm mechanisms of AMPs in C.albicans, providing the transcriptional profile of biofilms to open an interesting perspective for development of effective antifungal agents with antibiofilm efficacy in Candida.spp.ENAPictures, DmelCG8604, Adenosine 5' Phosphate, dAmph, amph, Procandida stellatoidea, APRTD, Phosphate Disodium, Adenylic Acid, Adenosine 2' Phosphate, Monilia stellatoidea, Antimicrobial Peptide, Picture, 5'-Adenosine monophosphate, PAdo, adenosine 5'-(dihydrogen phosphate), Damp, 6-(D-(2-amino-2-phenylacetamido))-3, damph, Musca <subgenus>, Dematium albicans, ampicillin acid, Films as Topics, Cinemas, AP, Adenosine-5'-monophosphoric acid, aminobenzylpenicillin, 5'-Adenylic, Musca <genus>, 6R)-6-{[(2R)-2-amino-2-phenylacetyl]amino}-3, Films as Topic, 5'-AMP, Films, ampicillin anhydrous, 3-DIMETHYL-7-OXO-4-THIA-1-AZABICYCLO[3.2.0]HEPTANE-2-CARBOXYLIC ACID, 2' Adenosine Monophosphate, Adenylate, Candida stellatoidea type I, adenosine phosphate, 5'-Adenylic acid, phosphate d'adenosine, Cinema, Movie, Motion, ampicilline, ampicilina, Ado5'P, Monilia albicans., Mycotorula albicans, 5'-Phosphate, Parasaccharomyces albicans, Antimicrobial, Anhydrous ampicillin, 2'-AMP, ADENOSINE MONOPHOSPHATE, Film, 2'-Adenosine Monophosphate, Adenosine 3'-Phosphate, (2S, Adenosine Phosphate Dipotassium, Motion Picture, Candida albicans var. stellatoidea, 5' Adenylic Acid, Syringospora albicans, 2'-Adenosine, 2'-Adenylic Acid, Peptide, Adenosine 3' Phosphate, ampicillin, AMP, Amp, 3-dimethyl-7-oxo-4-thia-1-azabicyclo(3.2.0)heptane-2-carboxylic acid, Procandida albicans, 2'-Adenylic, 5'-Adenylic Acid, fosfato de adenosina, CG8604, 3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid, Adenosine Phosphate, D-(-)-ampicillin, D-(-)-6-(alpha-aminophenylacetamido)penicillanic acid, antimicrobial peptide, 5R, Adenosine 5'-phosphate, Acid, 6R)-6-{[(2R)-2-amino-2-phenylethanoyl]amino}-3, Phosphate Dipotassium, Saccharomyces albicans, ABPC, Oidium albicans, adenosine-5'P, pA, Adenosine 5'-Phosphate, Phosphaden, 6R)-6-{[(2R)-2-AMINO-2-PHENYLETHANOYL]AMINO}-3, Disodium, 5'-O-phosphonoadenosine, Adenosine 2'-Phosphate, Movies, ampicillinum, Movies as Topic, Adenosine, Endomyces albicans, Adenosine Phosphate Disodium, Dipotassium, spindle cells, 2' Adenylic Acid, Candida stellatoidea, Movies as Topics, Monophosphate, adenosini phosphas, Adenylic acid, Myceloblastanon albicans, DAMPfalseThe Antimicrobial Peptide AMP-17 Derived from Musca do-mestica Inhibits Biofilm Formation and Eradiates Mature Bio-film in Candida Albicans2023-09-022023-09-02PRJNA879946