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& Microbiology, University of Nebraska Medical Centerhttps://www.ebi.ac.uk/ena/browser/view/PRJNA880848Staphylococcus aureusTo investigate biofilm-leukocyte crosstalk, we performed RNA-seq to evaluate how the S. aureus biofilm transcriptome was altered following exposure to G-MDSCs or macrophages in vitro. Interestingly, each immune cell type elicited a distinct transcriptional response. Overall design: RNA-sequencing was performed on biofilm co-cultured with G-MDSCs (3 replicates), macrophages (3 replicates), or G-MDSCs + macrophages (2 replicates). Biofilm only was also used as a control (2 replicates).pathogen:prioritypathogen:bacteriumxref:PubMed:36286525pathogenbacteriaENAmacrophagocyte, co-culture., MDSC, Cocultures, Transcriptome, Coculture Technique, Transcriptome Profile, Monocyte Derived Macrophages, typical plasmatocyte, Expression Profiles, Gene Expression Profile, Gene, Myeloid Derived Suppressor Cell, Profiles, Monocyte Derived, monocyte-derived macrophage, Cell, whole transcriptome, Gene Expression, Monocyte-Derived, macrophage, Suppressor Cells, Suppressor Cell, Expression Signatures, Gene Expression Signatures, Myeloid-Derived, Monocyte-Derived Macrophage, Cocultivation, Gene Expression Signature, Cocultivations, Biofilm, Expression Profile, agranular plasmatocyte, Co-culture, Monocyte-Derived Macrophages, Transcriptome Profiles, Macrophage, Coculture, Myeloid Derived Suppressor Cells, Co-cultures, Macrophages, Profile, Bone Marrow Derived Macrophages, Bone Marrow-Derived Macrophage, Signatures, Bone Marrow-Derived, lamellocyte, Expression Signature, Gene Expression Profiles, Cells, Myeloid-Derived Suppressor Cell, Bone Marrow-Derived Macrophages, Transcriptomes, Myeloid-Derived Suppressor, granulocyte, MDSCs, histiocyte, Signature, Co culturemacrophagocyte, co-culture., MDSC, Cocultures, Transcriptome, Coculture Technique, Transcriptome Profile, Monocyte Derived Macrophages, typical plasmatocyte, Expression Profiles, Gene Expression Profile, Gene, Myeloid Derived Suppressor Cell, Profiles, Monocyte Derived, monocyte-derived macrophage, Cell, whole transcriptome, Gene Expression, Monocyte-Derived, macrophage, Suppressor Cells, Suppressor Cell, Expression Signatures, Gene Expression Signatures, Myeloid-Derived, Monocyte-Derived Macrophage, Cocultivation, Gene Expression Signature, Cocultivations, Biofilm, Expression Profile, agranular plasmatocyte, Co-culture, Monocyte-Derived Macrophages, Transcriptome Profiles, Macrophage, Coculture, Myeloid Derived Suppressor Cells, Co-cultures, Macrophages, Profile, Bone Marrow Derived Macrophages, Bone Marrow-Derived Macrophage, Signatures, Bone Marrow-Derived, lamellocyte, Expression Signature, Gene Expression Profiles, Cells, Myeloid-Derived Suppressor Cell, Bone Marrow-Derived Macrophages, Transcriptomes, Myeloid-Derived Suppressor, granulocyte, MDSCs, histiocyte, Signature, Co culturefalseS. aureus biofilm transcriptome following G-MDSC or Macrophage co-cultureS. aureus biofilm transcriptome following G-MDSC or Macrophage co-culture2025-09-242022-10-07PRJNA880848GSE213381128036286525