ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR216/051/SRR21640351/SRR21640351.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR216/055/SRR21640355/SRR21640355.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR216/054/SRR21640354/SRR21640354.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR216/052/SRR21640352/SRR21640352.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR216/057/SRR21640357/SRR21640357.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR216/053/SRR21640353/SRR21640353.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR216/058/SRR21640358/SRR21640358.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR216/056/SRR21640356/SRR21640356.fastq.gzprimary200OKGenomicsmie universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA882428Plasmodium bergheipathogen:protozoanpathogenTo investigate the genome-wide binding sites of the transcriptional regulators, PbAP2-FG2 and AP2R-2 in female gametocytes of Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused PbAP2-FG2 (PbAP2-FG2::GFP) or GFP-fused AP2R-2 (AP2R-2::GFP) were harvested after treating the infected mice with sulfadiazine and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of PbAP2-FG2 and AP2R-2 were determined from the sequence data. Overall design: Mice were infected with PbAP2-FG2::GFP or AP2R-2::GFP and treated with sulfadiazine in their drinking water. Chromatins associated with GFP-tagged PbAP2-FG2 or AP2R-2 were IPed with anti-GFP antibody, and DNA fragments purified from the IPed chromoatins were sequenced by the Next Generation Sequencing. Using non-IPed samples as a control (INPUT), binding sites of PbAP2-FG2 and AP2R-2 were determined from the sequence data.protozoaENAfalsePbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [ChIP-seq]PbAP2-FG2 and AP2R-2 function together as a transcriptional repressor complex essential for Plasmodium female development [ChIP-seq]2025-09-242023-02-05PRJNA882428GSE2137755821