ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR217/039/SRR21701539/SRR21701539.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR217/035/SRR21701535/SRR21701535.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR217/041/SRR21701541/SRR21701541.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR217/034/SRR21701534/SRR21701534.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR217/037/SRR21701537/SRR21701537.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR217/033/SRR21701533/SRR21701533.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR217/040/SRR21701540/SRR21701540.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR217/036/SRR21701536/SRR21701536.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR217/038/SRR21701538/SRR21701538.fastq.gzprimary200OKGenomicsInstitute for Agricultural and Food Technology, Universitat de Gironahttps://www.ebi.ac.uk/ena/browser/view/PRJNA884105Prunus persicaWe charactarize the regulated miRNAs of Prunus persica to preventive treatment of PpPep2 1 µM endogenous peptide. The peptide treatment was applied to leaves and we sequenced the miRNAs after 1 and 24 h using untreated plants as control. miRNA profiles were generated by deep sequencing, in triplicate, using Illumina HiSeq 2500. Differential expression analysis of annotated pre_miRNA gene biotypes was performed with R package DESeq2 v1.26.0, considering significant epression values below FDR <0.05. We obtained 117,267,647 raw reads and we identified a total of 33 differential expressed miRNAs (DEM) belonging to 18 miRNA families upon PpPep2 application, accounting for 15% of the P. persica annotated miRNAs. miRNA regulation occurs principally one day after peptide treatment. The predicted mRNA targets and function of orthologous miRNAs are compatible with a regulation of PTI-related processes. Together with the transcriptomic response of P. persica to PpPep2 described at our previous RNA-Seq (Foix et al 2021), this suggests that miRNAs would have an active role in PTI regulation at these stages. Overall design: miRNA profiles of P. persica pretreated with PpPep2 after 1 and 24 h (t1, t24 h), untreated plants as control (t0).xref:PubMed:39684809ENAfalsemiRNAs have a role on Prunus persica response to topic application of PpPep2 endogenous peptidemiRNAs have a role on Prunus persica response to topic application of PpPep2 endogenous peptide2025-09-242024-12-21PRJNA884105GSE214135376039684809