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Lab, Medicine and Bioengineering, UC San Diego"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA886090"],"long_description":["The ischemic borderzone (BZ) is a geographically complex and biologically enigmatic interface separating poorly perfused infarct zones (IZ) from comparatively healthy remote zones (RZ).  BZ cellular and molecular mechanisms are not well understood because efforts to dissect it inevitably include RZ and IZ in uncontrolled proportions. Here, we use single-cell/nuclei RNA-sequencing, spatial transcriptomics, and multiplexed RNA fluorescence in situ hybridization (mFISH) to identify BZ cardiomyocytes (CMs) subsets. BZ1 (Nppa+Xirp2­-) forms a hundreds-of-microns-thick transitional layer adjacent to RZ, while BZ2 (Nppa+Xirp2­+) forms a tens-of-microns-thick layer that the IZ edge. BZ2 CMs have reduced CM cell contact colocalize with matricellular-protein-expressing myofibroblasts and upregulate focal adhesion-, sarcomere-, and cytoskeletal-genes.  Surprisingly, the transcriptional BZ emerges within an hour of injury and is inducible by non-ischemic fine-needle-trauma. We suggest that mechanical instability and “loss of neighbor” at the BZ edge are the dominant inducers of the BZ transcriptional response. Overall design: Gene expression profiling of 10x Genomic Chromium single nuclei RNA-Seq or Visium spatial transcriptomics from the left ventricular myocardium or short axis sections of healthy and injured adult male C57BL6 mouse hearts (8-12 weeks). Injuries include MI (permanent ligation of the LAD coronary artery), I/R-30 (30 minutes of ischemia followed by reperfusion), transaortic constriction, isoproterenol injection, and traumatic needle injection. Please note that the processed data files for GSM6613081, GSM6613087, GSM6613090 have been replaced on May 18, 2023 as the previous files were incomplete (missing spatial data folder and .h5 matrix file)."],"tag":["xref:PubMed:39086770"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Single Cell Spatial Transcriptomics Redefines the Borderzone induced by Myocardial Infarction and Mechanical Injury","description":"Single Cell Spatial Transcriptomics Redefines the Borderzone induced by Myocardial Infarction and Mechanical Injury","dates":{"last_updated":"2024-08-29","first_public":"2022-10-07"},"accession":"PRJNA886090","cross_references":{"GEO":["GSE214611"],"PubMed":["39086770"]}}