{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR223/055/SRR22355255/SRR22355255.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR223/056/SRR22355256/SRR22355256.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR223/053/SRR22355253/SRR22355253.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR223/054/SRR22355254/SRR22355254.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["Liver Cancer Institute, Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital Fudan University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA903800"],"scientific_name":["Mus musculus"],"long_description":["To understand how SIGLEC15 regulates CD8+ T cells in tumor microenvironment, we enriched CD8+ T cells using magnetic beads after tumor digestion. Collected CD8+ T cells were examined with flowcytometry analysis and were further loaded for sequencing analysis. Overall design: IgG (n=6) and anti-SIGLEC15 (n=6) treated percutaneous tumor grafts were constructed in C57 mice and collected in day19 after 2 weeks of intraperitoneal injection. Collected CD8+ T cells were stained for further examination in flowcytometry analysis. After confirmation, 3 samples of collected cell pelletes were mixed together and loaded for sequencing analysis."],"tag":["xref:PubMed:38988824"],"repository":["ENA"],"description_synonyms":["Cancer Microenvironments, Thymus-Dependent Lymphocytes, Microenvironment, T-Cells, p32, Thymus-Dependent, Leu2, T, Lymphocytes, RNA-seq., T-Lymphocyte, Tumor, Dietary Fiber, Cell, Thymus-Dependent Lymphocyte, T Cells, T Lymphocyte, T-Cell, Cancer Microenvironment, Cells, Whole Transcriptome Shotgun Sequencing, T Cell, CD8, Tumor Microenvironments, MAL, T Lymphocytes, Microenvironments, Thymus Dependent Lymphocytes, Lymphocyte, Cancer"],"name_synonyms":["Cancer Microenvironments, Thymus-Dependent Lymphocytes, Microenvironment, T-Cells, p32, Thymus-Dependent, Leu2, T, Lymphocytes, RNA-seq., T-Lymphocyte, Tumor, Dietary Fiber, Cell, Thymus-Dependent Lymphocyte, T Cells, T Lymphocyte, T-Cell, Cancer Microenvironment, Cells, Whole Transcriptome Shotgun Sequencing, T Cell, CD8, Tumor Microenvironments, MAL, T Lymphocytes, Microenvironments, Thymus Dependent Lymphocytes, Lymphocyte, Cancer"],"additional_accession":[]},"is_claimable":false,"name":"Functional Changes of CD8+ T Cells in Tumor Microenvironment after SIGLEC15 Blockade [bulk RNA-seq]","description":"Functional Changes of CD8+ T Cells in Tumor Microenvironment after SIGLEC15 Blockade [bulk RNA-seq]","dates":{"last_updated":"2025-09-24","first_public":"2024-08-10"},"accession":"PRJNA903800","cross_references":{"GEO":["GSE218409"],"taxon":["10090"],"PubMed":["38988824"]}}