ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/021/SRR22478821/SRR22478821_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/017/SRR22478817/SRR22478817_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/013/SRR22478813/SRR22478813_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/025/SRR22478825/SRR22478825_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/023/SRR22478823/SRR22478823_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/015/SRR22478815/SRR22478815_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/018/SRR22478818/SRR22478818_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/027/SRR22478827/SRR22478827_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/014/SRR22478814/SRR22478814_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/011/SRR22478811/SRR22478811_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/024/SRR22478824/SRR22478824_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/016/SRR22478816/SRR22478816_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/022/SRR22478822/SRR22478822_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/013/SRR22478813/SRR22478813_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/027/SRR22478827/SRR22478827_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/010/SRR22478810/SRR22478810_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/019/SRR22478819/SRR22478819_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/018/SRR22478818/SRR22478818_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/025/SRR22478825/SRR22478825_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/012/SRR22478812/SRR22478812_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/015/SRR22478815/SRR22478815_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/023/SRR22478823/SRR22478823_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/019/SRR22478819/SRR22478819_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/012/SRR22478812/SRR22478812_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/011/SRR22478811/SRR22478811_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/020/SRR22478820/SRR22478820_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/021/SRR22478821/SRR22478821_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/024/SRR22478824/SRR22478824_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/017/SRR22478817/SRR22478817_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/014/SRR22478814/SRR22478814_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/022/SRR22478822/SRR22478822_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/020/SRR22478820/SRR22478820_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/016/SRR22478816/SRR22478816_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/026/SRR22478826/SRR22478826_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/010/SRR22478810/SRR22478810_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR224/026/SRR22478826/SRR22478826_2.fastq.gzprimaryOK200GenomicsThe first affiliated hospital of Guangxi Medical Universityhttps://www.ebi.ac.uk/ena/browser/view/PRJNA907266Homo sapiensBronchopulmonary dysplasia (BPD) remains the most frequent chronic lung disease among infants, which involves multifactorial pathogenesis. Necroptosis represents a caspase-independent mode of programmed cell death, and its deregulation associates with lung diseases, but the mechanisms of necroptosis during BPD are indistinct. This work was conducted for unveiling the post-transcriptional regulatory mechanisms of necroptosis in BPD. Overall design: We sequenced messenger RNA (mRNA) (BPD, n=4 controls, n=4) and microRNA (miRNA) (BPD, n=5 controls, n=5) profiles in peripheral blood specimens. The GSE125873 dataset composed of mRNA profiling from 11 BPD individuals and 21 controls was gathered. Based upon the circBank and starBase databases, circRNA-miRNA-mRNA networks and a necroptosis-based subnetwork were built. The diagnostic efficacy of eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2) was assessed via receiver operator characteristic curve (ROC). EIF2AK2-relevant molecules were analyzed with weighted correlation network analysis (WGCNA), and then molecular classification was conducted through consensus clustering method.ENAMaterials, Genetic, Peripheral Blood, whole blood, PRKR, pkr1, Blood, Bronchopulmonary, Gene, INSDC_feature:gene, pkr, Cistrons, prkr, AI747578, eif2ak1, Reticuloendothelial System, Dysplasia, EIF2AK1, 2310047A08Rik, BPD., Material, AI467567, PBMC, PBMCs, Prkr, Genetic Materials, Cistron, Pkr, PKR, Tik, regulation of necroptosis, 4732414G15Rik, PPP1R83, Genetic MaterialMaterials, Genetic, Peripheral Blood, whole blood, PRKR, pkr1, Blood, Bronchopulmonary, Gene, INSDC_feature:gene, pkr, Cistrons, prkr, AI747578, eif2ak1, Reticuloendothelial System, Dysplasia, EIF2AK1, 2310047A08Rik, BPD., Material, AI467567, PBMC, PBMCs, Prkr, Genetic Materials, Cistron, Pkr, PKR, Tik, regulation of necroptosis, 4732414G15Rik, PPP1R83, Genetic MaterialfalsePost-transcriptional regulation of necroptosis gene EIF2AK2 in peripheral blood of bronchopulmonary dysplasiaPost-transcriptional regulation of necroptosis gene EIF2AK2 in peripheral blood of bronchopulmonary dysplasia2025-09-242024-03-30PRJNA907266GSE2191569606