ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/071/SRR23172771/SRR23172771_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/069/SRR23172769/SRR23172769_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/077/SRR23172777/SRR23172777_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/079/SRR23172779/SRR23172779_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/080/SRR23172780/SRR23172780_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/073/SRR23172773/SRR23172773_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/076/SRR23172776/SRR23172776_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/075/SRR23172775/SRR23172775_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/068/SRR23172768/SRR23172768_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/072/SRR23172772/SRR23172772_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/075/SRR23172775/SRR23172775_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/074/SRR23172774/SRR23172774_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/078/SRR23172778/SRR23172778_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/077/SRR23172777/SRR23172777_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/081/SRR23172781/SRR23172781_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/080/SRR23172780/SRR23172780_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/079/SRR23172779/SRR23172779_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/081/SRR23172781/SRR23172781_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/073/SRR23172773/SRR23172773_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/071/SRR23172771/SRR23172771_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/070/SRR23172770/SRR23172770_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/078/SRR23172778/SRR23172778_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/069/SRR23172769/SRR23172769_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/072/SRR23172772/SRR23172772_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/076/SRR23172776/SRR23172776_1.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/068/SRR23172768/SRR23172768_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/074/SRR23172774/SRR23172774_2.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR231/070/SRR23172770/SRR23172770_1.fastq.gzprimaryOK200GenomicsFred Hutchinson Cancer Centerhttps://www.ebi.ac.uk/ena/browser/view/PRJNA925929These samples are derived from tissues obtained from 4T1 spheroids transplantedorthotopically into the mammary fat pads of SRG OncoRats. 4T1 cell lines wereestablished by viral transduction that stably express either a non-targetingcontrol shRNA (NT4) or an shRNA targeting Angptl-7.4T1 cells were cultured as spheroids in suspension for 24-hours prior totransplantation in 2% matrigel. 600,000 cells in a 1:1 mixture of matrigel andDMEM/F12 were transplanted orthotopically into the right T4 fat pads of SRGrats.At day 27 post transplanation, primary tumors. Theprimary tumors were cut in half to expose the cross-section, and the tumorswere dissected to isolate the necrotic core region and the non-necrotic rimregion. Samples were snap frozen and sent to Genewiz-Azenta for RNA extraction,library prep, and next-gen sequencing.ENAfalseAngptl-7 knockdown RNA sequencing of 4T1 orthotopic transplants into rat mammary fat pads2024-01-042024-01-04PRJNA925929