ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/092/SRR23287092/SRR23287092.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/095/SRR23287095/SRR23287095.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/078/SRR23287078/SRR23287078.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/081/SRR23287081/SRR23287081.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/089/SRR23287089/SRR23287089.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/094/SRR23287094/SRR23287094.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/098/SRR23287098/SRR23287098.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/083/SRR23287083/SRR23287083.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/087/SRR23287087/SRR23287087.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/085/SRR23287085/SRR23287085.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/096/SRR23287096/SRR23287096.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/082/SRR23287082/SRR23287082.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/099/SRR23287099/SRR23287099.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/079/SRR23287079/SRR23287079.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/093/SRR23287093/SRR23287093.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/091/SRR23287091/SRR23287091.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/088/SRR23287088/SRR23287088.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/076/SRR23287076/SRR23287076.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/080/SRR23287080/SRR23287080.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/077/SRR23287077/SRR23287077.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/090/SRR23287090/SRR23287090.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/086/SRR23287086/SRR23287086.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/084/SRR23287084/SRR23287084.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR232/097/SRR23287097/SRR23287097.fastq.gzprimaryOK200GenomicsCSIChttps://www.ebi.ac.uk/ena/browser/view/PRJNA929954Homo sapiensxref:PubMed:37917179To investigate the MAFB-dependent genes in the SARS-COV-2 infection on human macrophages, we generated M-CSF and GM-CSF-dependent monocyte-derived macrophages in which MAFB has been knocked down by siRNA and then infected with SARS-CoV-2. Monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 for 6 days in the presence of GM-CSF or M-CSF, with cytokine addition every two days. Then, monocyte-derived macrophages were transfected with MAFB-specific siRNA or control siRNA, kept in culture for 6 hours, and then were exposed to SARS-CoV-2 (SARS-CoV-2 clinical isolate Gisaid EPI_ISL_1120962, corresponding to ancestral S D614G, at an MOI=1) or uninfected. After 36 hours, cells were lysed and RNA isolated for transcriptional analysis. We performed gene expression profiling analysis using data obtained from RNA-seq of 3 different donors. Overall design: Comparative gene expression profiling analysis of RNA-seq data for cells transfected with siMAFB (siMAFB_GM/siMAFB_M) or siControl (siCNT_GM/siCNT_M) exposed or not to SARS-COV-2 (MOI).ENAfalseTranscriptional effects of siRNA-mediated MAFB knockdown on SARS-CoV-2-infected human M-CSF and GM-derived macrophages.Transcriptional effects of siRNA-mediated MAFB knockdown on SARS-CoV-2-infected human M-CSF and GM-derived macrophages.2025-09-242024-02-11PRJNA929954GSE224131960637917179