{"database":"ENA","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Fastqsanger.gz":["ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR233/003/SRR23310603/SRR23310603_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR233/002/SRR23310602/SRR23310602_2.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR233/002/SRR23310602/SRR23310602_1.fastq.gz","ftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR233/003/SRR23310603/SRR23310603_1.fastq.gz"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"center_name":["College of Pharmacy, Pusan National University"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA930493"],"scientific_name":["Mus musculus"],"long_description":["Liver X receptor (LXR) is a critical regulator of cholesterol homeostasis, and inhibits TCR-induced proliferation by altering intracellular sterol metabolism. However, the mechanisms by which LXR regulates helper T cell subset differentiation in vivo remains unclear. Here we demonstrate here that LXR is a crucial regulator of follicular helper T (Tfh) cells in vivo. Both mixed bone marrow chimera and antigen-specific T cell adoptive co-transfer studies show a specific increase in Tfh cells among LXRβ-deficient CD4+ T cell population in response to immunization and LCMV infection. Mechanistically LXRβ-deficient Tfh cells express increased levels of TCF-1 but comparable levels of Bcl6, CXCR5, and PD-1 in comparison with LXRβ-sufficient Tfh cells. Loss of LXRβ confers inactivation of GSK3β induced by either AKT/ERK activation or Wnt/β-catenin pathway, leading to elevated TCF-1 expression in CD4+ T cells. Conversely, ligation of LXR represses TCF-1 expression and Tfh cell differentiation in both murine and human CD4+ T cells. Treatment with an LXR agonist significantly diminishes Tfh cells and the levels of antigen-specific IgG upon immunization. These findings unveil a novel cell-intrinsic regulatory function of LXR in Tfh cell differentiation via controlling GSK3β-TCF1 pathway, which might be a promising target for pharmacological intervention in Tfh-mediated diseases. Overall design: mRNA profiles of follicular helper T cells from wild type and Nr1h2-/- mice were generated by deep sequencing, singleplicate, using Illumina Truseq"],"tag":["xref:PubMed:36802434"],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Liver X receptor controls follicular helper T cell differentiation via repression of TCF-1","description":"Liver X receptor controls follicular helper T cell differentiation via repression of TCF-1","dates":{"last_updated":"2025-09-24","first_public":"2023-02-07"},"accession":"PRJNA930493","cross_references":{"GEO":["GSE224303"],"taxon":["10090"],"PubMed":["36802434"]}}