ENAapplication/xmlftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/021/SRR23400621/SRR23400621.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/010/SRR23400610/SRR23400610.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/015/SRR23400615/SRR23400615.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/004/SRR23400604/SRR23400604.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/026/SRR23400626/SRR23400626.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/022/SRR23400622/SRR23400622.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/011/SRR23400611/SRR23400611.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/009/SRR23400609/SRR23400609.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/000/SRR23400600/SRR23400600.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/005/SRR23400605/SRR23400605.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/020/SRR23400620/SRR23400620.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/012/SRR23400612/SRR23400612.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/016/SRR23400616/SRR23400616.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/027/SRR23400627/SRR23400627.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/023/SRR23400623/SRR23400623.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/019/SRR23400619/SRR23400619.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/001/SRR23400601/SRR23400601.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/008/SRR23400608/SRR23400608.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/018/SRR23400618/SRR23400618.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/007/SRR23400607/SRR23400607.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/002/SRR23400602/SRR23400602.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/013/SRR23400613/SRR23400613.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/024/SRR23400624/SRR23400624.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/014/SRR23400614/SRR23400614.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/003/SRR23400603/SRR23400603.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/017/SRR23400617/SRR23400617.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/006/SRR23400606/SRR23400606.fastq.gzftp://ftp.sra.ebi.ac.uk/vol1/fastq/SRR234/025/SRR23400625/SRR23400625.fastq.gzprimaryOK200GenomicsThomas Winkler, Genetics, FAU Erlangenhttps://www.ebi.ac.uk/ena/browser/view/PRJNA934585Mus musculusxref:PubMed:37182210The clonal selection theory describes the key features of adaptive immune responses of B and T cells. For αβ T cells and B cells antigen recognition and selection principles are known at a detailed molecular level. The precise role of the antigen receptor in γδ T cells remains less well understood. To better understand the role of the γδ T cell receptor (TCR), in particular its specificity for thymic selection of γδ T cells and for γδ T cell biology in general, we generated a novel orthotopic TCRδ transgenic mouse model. We demonstrate a multi-layered functionality of γδ TCRs and diverse roles of CDR3δ-mediated selection during γδ T cell development. Whereas epithelial populations using Vγ5 or Vγ7 chains are affected to only minor extend in their biology in the presence of a single TCRδ chain, pairing with Vγ1 positively selects subpopulations of γδ T cells with distinct programs in several organs, thereby distorting the repertoire. In conclusion, our data support dictation of developmental tropism together with adaptive-like recognition principles in a single antigen receptor. Overall design: Murine γδ T cells were isolated from the spleen via Fluorescence-activated cell sorting (FACS) according to the presence of CD3 and γδTCR and the absence of αβTCR, B220 and CD11c, and analyzed using scRNAseq for transcriptome and V(D)J repertoire analysis.ENAfalseA monoclonal Trd chain supports the development of the complete set of functional γδ T cell lineages (GEX)A monoclonal Trd chain supports the development of the complete set of functional γδ T cell lineages (GEX)2025-09-242023-03-06PRJNA934585GSE2250321009037182210