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Functional Genomics Common Research Resource, Department of Psychiatry and Neurology Department of Psychiatry, University of California, Los Angeles"],"full_dataset_link":["https://www.ebi.ac.uk/ena/browser/view/PRJNA940089"],"scientific_name":["Mus musculus"],"tag":["xref:PubMed:37973910"],"long_description":["We generated adeno-associated Cre viral vectors with improved astrocyte-specific expression by including a cassette of miRNA targeting sequences (4x6T) to decrease off-target expression. We delivered these viruses in Ribotag mice using the PHP.eB serotype, either intracortically or systemically. We compared these samples with mice that received intracortical AAV2/5::GfaABC1D-Cre and transgenic Aldh1l1-CreERT2/Ribotag mice. We sequenced whole-tissue RNA as well as ribosomally-loaded astrocyte-enriched mRNA to assess cell-type specificity of viruses as well as virus-related transcriptomic changes. Overall design: Four cohorts were generated: Aldh1l1-CreERT2/Ribotag transgenic mice (control mice with highly astrocyte-specific labeled ribosomes) AAV2/5::GfaABC1D-Cre intracortical injection mice (standard astrocyte-enriched virus) PHP.eB::GfaABC1D-Cre-4x6T intracortical injection mice and PHP.eB::GfaABC1D-Cre-4x6T systemic injection mice. Each cohort included 4 mice, and each mouse had both input (whole-cortex) and immunoprecipitated (ribosomally loaded, astrocyte-enriched) mRNA samples."],"repository":["ENA"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional analysis of murine cortical astrocytes with adeno-associated viral transduction with and without 4x6T miR cassette, vs transgenically labeled astrocytes","description":"Transcriptional analysis of murine cortical astrocytes with adeno-associated viral transduction with and without 4x6T miR cassette, vs transgenically labeled astrocytes","dates":{"last_updated":"2025-09-24","first_public":"2023-03-06"},"accession":"PRJNA940089","cross_references":{"GEO":["GSE226366"],"taxon":["10090"],"PubMed":["37973910"]}}